Final 03 - Name 12 Lac Operon(30 points page 5 A Given the...

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Name: page 5 12. Lac Operon (30 points) A. Given the genotypes, fill in the appropriate phenotypes for LacZ and LacY. The inducer is lactose or IPTG. Phenotypes LacZ expression LacY expression Genotype no inducer inducer no inducer inducer 1 I+O+Z+Y+ 2 I S O+ Z+ Y- 3 I S O + Z- Y+ / F’I-O c Z+Y- 4 I+O c Z+Y-/ F’ I-O + Z + Y - 5 I-O C Z+Y + / F’ I S O + Z - Y - B. Name one strain that indicates that O C is epistatic to I S , and what data tells you that? #5, lacZ and lacY expression (credit given for either) #3 lacZ expression C. A new gene is identified that regulates the Lac operon, called Gene T. An allele Gene T-2 was analyzed. We know that Gene T+ acts in the glucose, ADC1, CRP pathway. The assays of Gene T+ function are done in a I- cell, with glucose present. (The phenotypes would be complicated by reporting LacZ expression with no glucose and with glucose column. However, you can figure this out using this data alone). LacZ expression 1. GeneT+ - 2. GeneT-2 + 3. GeneT-2/ Gene T+ + 4. ADC1* + 5. ADC1* GeneT-2 + 6. ADC1- - 7 . ADC1- Gene T-2 + i. Is Gene T-2 recessive or dominant (circle one) ii. Draw the glucose, Gene T+,ADC1,CRP, LacZ, pathway, with gene order and sign of regulation (bar or arrow) Glucose ADC1+ GeneT+ CRP LacZ 4pt order, 2 pt each bar/arrow=12pt overall iii. Why is the combination of strains 2, 4 and 5 not informative for trying to determine the order of function of GeneT+ and ADC1+?
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