exam3 - Name UGAMyID BCMB 3100(Medlock Fall 2005 Exam 3(120...

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Name: ______________________________ UGAMyID: _______________ BCMB 3100 (Medlock) Fall 2005 Exam 3 (120 points) 1. (15 points) (A) Describe the steps of glycolysis in which ATP is used or synthesized. Describe each step assuming that 1 molecule of glucose is metabolized. Include either the enzyme involved or the substrate and product of the reaction. (B) Describe two ways in which glycolysis is regulated. Include the activator or inhibitor, the type of regulation, and the enzyme or substrate and products of the reaction. 2. (10 points) (A) Patients in shock often suffer from lactic acidosis due to a deficiency on oxygen. How does a lack in oxygen lead to a build up of lactic acid? Are these patients still producing ATP if glucose is available? Explain your answer. (B) Pyruvate is the product of glycolysis and acetyl CoA is the initial substrate of the citric acid cycle. How are these two pathways connected? What is the overall reaction of this step?
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Name: ______________________________ UGAMyID: _______________ 3. (15 points) Below you will find a chart with each of the eight steps of the citric acid cycle. Note that they are out of order. Fill in the first column with which step (1-8) of the citric acid cycle this reaction occurs. Also include in the chart what type of reaction occurs and any other compounds or coenzymes that are used or produced. Step # Reaction Type of reaction Other compounds used Other compounds produced Succinate to fumarate L -malate to oxaloacetate Citrate to isocitrate α -ketoglutarate to succinyl CoA Oxaloacetate and acetyl CoA to citrate Isocitrate to α - ketoglutarate Succinyl CoA to succinate Fumarate to L -malate 4. (10 points) (A) The conversion of citrate to isocitrate has a Gº’ of +8.4 KJ/mol yet is proceeds in the
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