biochem 466homework6key

biochem 466homework6key - Homework 6 Key 2 A PDB 1d8h...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Homework 6 Key 2. A) PDB 1d8h and/or 1d8i Cell. 1999 Nov 24;99(5):533-43. Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus. Lima CD, Wang LK, Shuman S. The active site is all about charge interaction. One side of the tube (termed “floor”) has negatively charged acidic residues that coordinate a metal (?Mg++). The Mg++ helps in hydrolysis in a manner similar to how it works in MANY phosphodiester bond cleavages. The Mg++ has two jobs: 1) it activates the gamma phosphate to start the reaction, and 2) it stabilizes the transition state. As you know from first semester, this makes the rate faster. The other side of the tube (the roof) has positively charged residues that help position the phosphate for the reaction. B) PDB 1cko Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1505-10. Structure of a complex between a cap analogue and mRNA guanylyl transferase demonstrates the structural chemistry of RNA capping. Hâkansson K, Wigley DB. In the reaction depicted in Figure 4, Mg++ is doing the same kind of job that it does in 2A, except
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This homework help was uploaded on 04/02/2008 for the course BIOC 466 taught by Professor Hallick,dieckmann,horton during the Spring '08 term at Arizona.

Page1 / 2

biochem 466homework6key - Homework 6 Key 2 A PDB 1d8h...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online