Lab 4.pdf - Lab 5 Lab 5 Microscopes and Cells Objectives 1 Learn the structures and functions of intracellular organelles 2 Be able to operature and

Lab 4.pdf - Lab 5 Lab 5 Microscopes and Cells Objectives 1...

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9/16/2019 Lab 5 1/28 Lab 5 Microscopes and Cells Objectives 1. Learn the structures and functions of intracellular organelles. 2. Be able to operature and focus a microscope. 3. Be able to prepare slides and identify structures of organisms present on prepared slides. Student Learning Outcomes Learning Objective Activity/Assignment that Addresses Objective Critical Thinking Compare and contrast prokaryotic and eukaryotic cells; compare and contrast protists, plant cells, and animal cells Communication Skills Written -- prelab assignment Oral -- information; interaction and communication with team members and laboratory instructor Teamwork Students will be completing explorations as members of a lab group/team. Personal Responsibility completion of prelab activities; coming to class prepared Cell Fundamental Unit of all living systems Cell Theory: all originate from and consist of cells Microscopy Microscopes magnify images and enable students and researchers to visualize cells and structures that would otherwise be invisible to the naked eye. A person with good eyesight, who knows what he/she is looking can see objects that are about 0.1 mm in length. However, no internal structure or detail of this 0.1 object would be visible. The space shown in red is 1 mm, so theoretically, you should be able to see something 1/10th that width. The compound light microscope is used in the introductory biology labs. It is a light microscope, because visible light is the light source. Microscopes with light sources that have a smaller wavelength (eg. ultraviolet light) are capable of greater resolution, and higher magnification, than light microscopes. The light source is usually a lamp that is built into the scope. The condenser is a structure on the microscope that functions to concentrate light on the specimen. The microscope is compound because it has multiple lenses. The objective lens is located above the stage/specimen, and magnifies the image, and the ocular lens, that you look through, further magnifies the image. Compound light microscopes can magnify an image up to about 1000 times. Electron microscopes, which use electrons (very low wavelength) as a light source, can magnify structures up to 200,000 times. For a summary of types of microscopes, and their functions, click here.
9/16/2019 Lab 5 2/28 Resolution and level of magnification are important aspects of microscopy and in selecting a microscope. The microscopes you will be using are binocular compound light microscopes. Standard magnification of the ocular lenses is 10 times. When looking at your specimen, the temptation is to close one eye and use only one of the ocular lenses. Those ocular lenses can be adjusted to the width of your eyes. We recommend that you learn to use the microscopes with both eyes. The nosepiece houses the objective lenses. There are four objective lenses attached to the nosepiece.

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