PCR and DNA.pdf - Friday 8:46 AM Recombinant DNA DNA from 2 sources Biotechnology ways to manipulate biology with different tools Genetic engineering

PCR and DNA.pdf - Friday 8:46 AM Recombinant DNA DNA from 2...

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Recombinant DNA- DNA from 2 sources Biotechnology- ways to manipulate biology with different tools Genetic engineering- alteration of genetic code to modify an organism Plasimid- alternate set of DNA used in certain situations ^ small double DNA molecule ^carries accessory genes that aren't usually present in a bacterial chromosomes (Bacteria or eukaryote plasmid (yeast)) Gene cloning- copying genes Cloning vector- bacteria mostly used; DNA molecule carries certain segments in DNA to the host Fast replication; gets recombinant DNA into a host= bacterial cloning vectors We use the same restriction enzyme to cut out a gene of interest and a plasmid bc it cuts the dna in both places DNA ligase= binding together Transformation is when we do cloning w/ bacteria ^when the bacteria fills up the plasmids Genomic library- to store information about the entire genome PCR Steps DENATURIZATION ANNEALING ELONGATION Gel electrophoresis- Southern blotting- You'd be able to look at the specific nucleotide sample that you're

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