Final'07PracticeKey

Final'07PracticeKey - R L Rodriguez Instructor BlS101-001...

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R. L. Rodriguez, Instructor BlS101-001, Spring 2008, Practice Final Exam 1. Which of the following statements is true regarding gene expression? Circle the correct answer(s). (6 pts.) D. @ aA) Proteins are synthesized by the transfer of the polypeptide chain to the amino terminus of the next amino acid B. The first step is the association of mRNA with ribosomes C. Involves proof-reading of the mRNA Prokaryotic RNA usually undergoes nuclear processing The 3' end of mRNA corresponds to the carboxyl terminus of the protein 2. Which of the following statements is true about the splicing of introns from hnRNA? Circle the correct answer(s). (6 pts.) (ry The splicing of introns must obey the GU/AG rule The 3'end of the exon forms a phosphodiester bond with the branch point "A" in the intron The 5' end of the intron forms a phosphodiester bond with the 5' end of the exon The splicosome consists of proteins and small pieces of RNA called ubiquitous RNA. The formation of phosphodiester bonds requires a polymerase activity in the splicosome C. @ E.
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Name 3. The Wobble Hypothesis states that the third base at the 5' side of the anticodon loop can hydrogen bond with certain noncomplementary bases. The Wobble rules are shown below. Using these rules, determine the minimum number of anticodons (i.e. tRNAs) that can recognize all six leucine codons. (Show your work). (15 p*.) Base at 5' end of Anticodon Base at 3' end of codon- I (deaminated A) A, U, C G C,U U A.G CG AU Codons: 5'UUA UUG CUU CUC CUA CUG3' Anticodons' AA v GAl. ? GAc 4. A novel microorganism, Bacillus lactus, is isolated from the mammary gland of a lactating cow. Like E coli,this organism can utilize lactose as a carbon source. Unlike E. coli, however, B.lactus is naturally constitutive for the B-galactosidase enzyme, expressing it at high levels even when grown in glucose. To investigate the similarities between this new microbe and normal E. coli, researchers mutagenized Bacillus lactis and isolated six mutants that can no longer use lactose as a carbon source (lac-). These mutants were analyzed using the complementation table shown below.
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Final'07PracticeKey - R L Rodriguez Instructor BlS101-001...

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