Final'08PracticeKey

Final'08PracticeKey - 2l l y 0 l S I o I Do o t f r n E x a...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
*"-"0lSIoI-Dl 2oot'frn"l ExanrKey Ue"sio t- l. The figure below shows a single nucleotide polymorphism (SNP) between two alleles that creates a BamHI restriction fragment lengthpolymorphism (RFLP) in a l0 kb stretch of chromosomal DNA. The RFLP can be detected by Southern blot analysis using a radiolabeled probe complementary to this region. Because this SNP is associated with the Sickle Cell Anemia, individuals can be screened for this autosomal recessive disease. Hornolog 1 Homolog 2 Three individuals submitted their DNA for genetic testing to determine their risks for the disease. The Southern blot analysis reveals the following bands. Individual I l.5kb Individual 2 l.5kb ana Z4UA 4 Individual 3 4kb (a) Which individualwill definitely get the disease? (5 pts.) S 3 (b) Which individual is a carrier of the disease allele? (5 pts.) 4 L (c ) Which individual is not a carrier of the disease allele? (5 pts.) ++ | (d) If a probe corresponding to the 8kb region were used instead of the one corresponding to the 6.5 kb region, what new banding pattern would be indicative of a carrier of the disease. Circle the correctanswer(s) below. (5 pts.) r.skb @
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Name 2. The Wobble Hypothesis states that the third baseat the 5' side of the anticodon loop can hydrogen bond with certain noncomplementary bases. The Wobble rules are shown below. Using these rules, determine the minimum number of anticodons (i.e. tRNAs) that can recognize all six arginine codons. (Show your work). (15 pts.) Base at 5' end of Anticodon I (deaminated A) G U C A Base at 3' end of codon- A , U , C C , U A , G G U Codons: Anticodons: CGU CGA AGG3' 3. A novel microorganism, Bacillus lactus, is isolated from the mammary gland of a lactating cow. Like E. coli,this organism can utilize lactose as a carbon source. Unlike E. coli, however, B. lactus rs naturally constitutive for the S-galactosidase enzyme, expressing it at high levels even when grown in glucose. To investigate the similarities between this new microbe and normal E. coli, researchers
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern