Final'08PracticeKey - 2l l y 0 l S I o I Do o t f r n E x a...

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*"-"0lSIoI-Dl 2oot'frn"l ExanrKey Ue"sio t- l. The figure below shows a single nucleotide polymorphism (SNP) between two alleles that creates a BamHI restriction fragment length polymorphism (RFLP) in a l0 kb stretch of chromosomal DNA. The RFLP can be detected by Southern blot analysis using a radiolabeled probe complementary to this region. Because this SNP is associated with the Sickle Cell Anemia, individuals can be screened for this autosomal recessive disease. Hornolog 1 Homolog 2 Three individuals submitted their DNA for genetic testing to determine their risks for the disease. The Southern blot analysis reveals the following bands. Individual I l.5kb 2 l.5kb ana Z4UA 4 3 4kb (a) Which individual will definitely get the disease? (5 pts.) S 3 (b) Which individual is a carrier of the disease allele? 4 L (c ) Which individual is not a carrier of the disease ++ | (d) If a probe corresponding to the 8kb region were used instead of the one corresponding to the 6.5 kb region, what new banding pattern would be indicative of a carrier of the disease. Circle the correct answer(s) below. (5 pts.) r.skb @
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Name 2. The Wobble Hypothesis states that the third base at the 5' side of the anticodon loop can hydrogen bond with certain noncomplementary bases. The Wobble rules are shown below. Using these rules, determine the minimum number of anticodons (i.e. tRNAs) that can recognize all six arginine codons. (Show your work). (15 pts.) Base at 5' end of Anticodon I (deaminated A) G U C A Base at 3' end of codon- A,U,C C,U A,G G U Codons: Anticodons: CGU CGA AGG3' 3. A novel microorganism, Bacillus lactus, is isolated from the mammary gland of a lactating cow. Like E. coli,this organism can utilize lactose as a carbon source. Unlike E. coli, however, B. lactus rs naturally constitutive for the S-galactosidase enzyme, expressing it at high levels even when grown in glucose. To investigate the similarities between this new microbe and normal E. coli, researchers
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This note was uploaded on 07/03/2009 for the course BIS BIS 101 taught by Professor Sanders during the Spring '09 term at UC Davis.

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Final'08PracticeKey - 2l l y 0 l S I o I Do o t f r n E x a...

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