Bio1AL_Sum09_lab6_prelab

Bio1AL_Sum09_lab6_prelab - DNA electrophoresis,...

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A25 – Summer 2009 DNA electrophoresis, Complementation, and GMB II Pre-lab. Due at the start of lab. Name GSI & Sect # Station # To answer these questions read all of the lab material on constructing recombinant DNA (pages 60-86) even though you are only doing pages 81-84 for lab 6. You also need to read about complementation (pages 107-114) even though you are only doing pages 112-114. You also need to read about GMB II (pages 141-153). Write legibly. You will NOT be admitted to lab unless you turn in this completed pre-lab at the start! Please write your answers . NOTE WELL : You will not be admitted to your lab unless you turn in this completed pre-lab at the beginning of your lab! The genetics portion is typically very difficult for many students. Your instructor(s) will review the material in lab and lecture. 1) Define the following terms: autosomal chromosomes sex chromosomes genetically linked genes X-linked genes 2) Name two purposes of the loading dye? 3) Migration of DNA in an agarose gel varies depending upon the size and conformation of the DNA. A mixture of three DNA conformations, all having a size of 4,500 base pairs, was loaded into a well and the sample was electrophoresed. The banding pattern of the DNA is shown below. Note that the band that corresponds to linear DNA
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This note was uploaded on 07/04/2009 for the course BIO 1al taught by Professor Pederson during the Summer '08 term at Berkeley.

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Bio1AL_Sum09_lab6_prelab - DNA electrophoresis,...

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