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Bio1AL_Sum09_lab3_wksht - Transformation and Enzyme...

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A11 - – Summer 2009 Transformation and Enzyme Worksheet. Due at the start of your next lab. Name GSI & Sect # Station # 1. Did you label the bottom of both of your plates and then tape the two plates together using small pieces of tape? Circle one: Yes / No Did you place them in the tray upside down (agar up) at the instructors bench? Yes/ No 2. a) Graph your results from Part I- Relationship between enzyme concentration and enzyme activity . You must convert Optical Density into µ moles of maltose. 0 100 200 300 400 2. b) Would you expect a linear or non-linear dependence based upon how enzymes function as catalysts? Explain in terms of a simple model for enzyme catalysis. See the tutorial if needed.
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A12 - – Summer 2009 3. a) Graph the results of one set of data for part II-A. DO NOT AVERAGE CLASS DATA – instead pick the best set of data (or plot your own if you did this part). 0 25 50 75 100 3. b) What is the temperature optimum of salivary amylase? How does this compare to body temperature? (include units) 4. Explain the basis for a temperature optimum in terms of enzyme structure and kinetics.
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