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Bio1AL_Sum09_lab1_lecture

Bio1AL_Sum09_lab1_lecture - Bio 1AL Lab Lecture Outline 2 I...

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Bio 1AL Lab Lecture Outline # 2 June 24 th , 2009 I. Safety- common sense II. Light Spectroscopy A. Overview B. Identification – Absorption Spectrum C. Quantification – (A = cpZ) D. Blank – why? E. Exercise – make absorption spectrum red dye (H 2 O) & quantification III. Micropipetter A. Why – measure small volumes precisely B. Use – tips & points of resistance i) P20 – (1-20 μ L), P200 – (20-200 μ L), P1000 – (200-1000 μ L) C. Do's & Don'ts D. Sterile technique E. Exercise – weigh various volumes w/ a balance IV. Cloning & Ligation note: steps will become clearer each week. A. DNA structure B. Overview- Isolate DNA of interest and vector DNA C. Restriction Digest (Cut) D. Ligate, Transform, Screen (various ways) and analyze! For example – Insulin gene Host cells with vector Human tissue fragment cells to release DNA fragment cells to release DNA follow steps to isolate vector DNA follow steps to isolate DNA Isolated vector DNA Isolated source DNA Restriction digestions Mix & Anneal Ligate: 3 possible results Transform & Screen
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V. Microscope
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