Bio100LW_cauliflower lab.doc

Bio100LW_cauliflower lab.doc - Bio100LW Q5 Online Databases...

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Bio100LW Q5 Online Databases Worksheet Spring 2009 Worksheet for Lab, Week 9 Brassica Evolution Cauliflower Flower Abnormality at the Molecular Level Please complete the following information and upload the file to Q5 Online Databases in your lab section DropBox. 1. What is the locus tag for the CAL gene of Arabidopsis thaliana ? Please include locus tag prefix and number. AT1G26310 2. On what chromosome is the CAL gene in Arabidopsis thaliana 1 3. How many exons does the Arabidopsis thaliana CAL gene have? 8 4. What stock name would you use to order seeds from the TAIR stock center of the double mutant of Arabidopsis thaliana (ap-1, cal-1)? CS6161 5. What are the three codon sequences for Leucine, Tryptophan and Translation STOP in Arabidopsis thaliana ? Leucine TTA TTG CTT CTC CTA CTG Tryptophan TGG Translation STOP TAA TAG TGA 6. Why does Arabidopsis thaliana have 7 genome sequences?
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Bio100LW Q5 Online Databases Worksheet Spring 2009 It shows us the different chromosomes (1-5) and the plastid and mitochondrion sequences 7. What gene, besides CAULIFLOWER, frequently appears in the sequences with the highest matches to CAL of Arabidopsis thaliana ? APETALA1 8. Under the description of the MADS domain it states that it ”binds DNA and exists as hetero and homo-dimers” (NCBI). What can this tell us about a protein containing a MADS box domain? Important in homeotic regulation for plants 9. Paste the image of Polyketide Synthase from the Protein Data Base here. 10. Find the page for Alcohol Dehydrogenase. What month was Alcohol Dehydrogenase the Molecule of the Month? January 2001 11. What does Alcohol Dehydrogenase do for us? It turns alcohol into acetaldehyde 12. Paste the Alignment of the BoCAL, BobCAL and BoiCAL protein sequences using CLUSTERW here using print screen (PrtScn). CLUSTAL 2.0.10 multiple sequence alignment
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Bio100LW Q5 Online Databases Worksheet Spring 2009 uniprot_BobCal_642592 M G R G R V E M KR I E N K I N R Q V T F S KRR A G LL KK A H E I S IL C D A E V S LIVF SH 50 uniprot_BoiCal_1561783 M G R G R V E M KR I E N K I N R Q V T F S KRR A G LL KK A H E I S
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Bio100LW_cauliflower lab.doc - Bio100LW Q5 Online Databases...

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