practice Final Exam

practice Final Exam - LS3 Final Exam INSTR L Johnson...

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LS3 Final Exam INSTR: L. Johnson Thursday, Dec. 12, 2002 NAME:_________________________________ (last) (first) I.D. #__________________________________ INSTRUCTIONS 1. This exam is 14 pages long. PAGE VALUE SCORE Before starting make sure you have all the pages. 2 19 2. Be sure to write clearly. Only 3 12 legible answers will be graded. 4 22 5 30 6 17 7 17 8 18 9 21 10 18 11 26 200 Lab 20 Total 220
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2 NAME:_________________________________ ID# ________________________ (last) (first) Part A. New Material (100 pts) (8 pts) 1. Restriction enzymes recognize palindromic sequences and cut both strands of DNA at the same sequence. Consider the four enzyme recognition sites shown below and the types of fragments they generate. Restriction Enzyme Recognition Site BspE I 5’ T^CCGGA 3’ Eag I 5’ C^GGCCG 3’ Apa I 5’ GGGCC^C 3’ NgoM I 5’ G^CCGGC 3’ a) Which enzymes produce a 4 base sticky ends? b) If you wanted to clone a fragment which was digested with one enzyme into a vector digested with another enzyme, which two enzymes above could be used (i.e. have compatible ends for cloning)? (11 pts) 2. a) Hybridization is used in many molecular biology techniques. Why is this such a powerful technique? b) Describe in general what an antibiotic is and how it is used during cloning procedures. c) Describe in general what an antibody is and how it is used during cloning procedures.
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3 NAME:_________________________________ ID# ________________________ (last) (first) (12 pts) 3. You have succeeded in isolating a novel human virus and proceed to create a restriction map using two enzymes, Sal I and Bam HI. After digestion and electrophoresis you obtain the results shown below. However, when you try to determine the restriction map you find there are two possible maps consistent with the data you obtained. To distinguish which map is correct, you repeat the digestions and electrophoresis, but this time you transfer the DNA to nitrocellulose and perform a Southern analysis, using the 4 kb restriction fragment from the Bam HI + Sal I digest as a probe. Using both sets of data, determine the correct restriction map. Agarose Gel Southern Analysis BamHI BamHI SalI Sal I Bam HI SalI Sal I Bam HI 13 10 10 8 8 7 7 7 6 6 5 4 4 4 3 3 2 1
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4 NAME:_________________________________ ID# ________________________ (last) (first) (12 pts) 4. Meselson and Stahl performed a series of classic experiments looking at DNA replication. a) What were the two models they were trying to distinguish between? b) What did they do in order to separate newly synthesized DNA from the template DNA? c) These experiments were done using bacteria as a model system. How were their confirmed for eukaryotic cells? (10 pts) 5.
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practice Final Exam - LS3 Final Exam INSTR L Johnson...

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