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lec01a.ppt [Compatibility Mode] - Course Materials !...

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Unformatted text preview: Course Materials ! Fundamental Molecular Biology " Lizabeth Allison, 1 st edition ! LS3 lab manual ! LS3 reader " Course Reader Material, 1141 Westwood Blvd. ! Web site " http://www.lsic.ucla.edu/ Discussion/Lab Week 1 3 5 7 and 10 you will meet ! Week 1, 3, 5, 7, and 10 you will meet in your lab room. ! Week 2, 4, 6, and 8 you will meet in discussion room. ! Week 9 is Thanksgiving (only Tuesdays sections will meet to make up Nov 11 holiday) Online Quizzes Due every week on Mondays at 12 ! Due every week on Mondays at 12 noon (6 total) ! Start next Friday ! One chance for each quiz- have your notes available ! 5 pts each Grading Two midterms: 200 pts ! Two midterms: 200 pts ! Lab: 100 pts ! Quizzes: 30 pts ! Final: 200 pts Overview of Course ! I " Structure of DNA, RNA, proteins " Enzymes and antibodies " Prokaryotic transcription, gene regulation " Prokaryotic translation ! II " DNA Replication, repair " Recombinant DNA technology Eukaryotic transcription " Eukaryotic transcription ! III " Splicing " Epigenetics and genomics LS3 Labs ! Goal: " Learn techniques commonly used in molecular biology labs molecular biology labs " Learn how to use bioinformatics tools and databases " Answer various questions in biology How many different polypeptides are found in a protein and what are their sizes? in a protein and what are their sizes? How do living organisms respond to a change in environment? How can DNA be used to determine how related species or individuals are? Lab ! Lab I: SDS gel electrophoresis Use polyacrylamide gel electrophoresis Hemoglobin: 2 ! , 2 " " Use polyacrylamide gel electrophoresis to determine number and size of subunits in a protein " Basic technique essential in molecular biology and biochemistry How can we detemine the size and number of polypeptides in a protein? hemoglobin protein? ! Purify protein ! Dissociate into separate polypeptides (detergent: SDS) ! Separate using polyacrylamide gel electrophoresis SDS Polyacrylamide gel forms matrix that separates proteins based on size Lab 1 ! Youll be given unknown protein of given MW " Denature " Separate subunits on polyacrylamide gel " Determine how many of each subunit...
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This note was uploaded on 07/08/2009 for the course LIFESCI LS 3 taught by Professor Paulolague during the Fall '09 term at UCLA.

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lec01a.ppt [Compatibility Mode] - Course Materials !...

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