LEC02A.PPT [Compatibility Mode]

LEC02A.PPT [Compatibility Mode] - Last time: ! Griffith...

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Unformatted text preview: Last time: ! Griffith experiment and Avery et. al. experiments, ! Transforming agent is DNA ! Not accepted universally ! Did open scientific thought to the possibility that DNA may be the possibility that DNA may be the chemical associated with “genes” ! Stimulated experiments in other systems to confirm or refute this finding Hershey Chase Experiment ! Hershey-Chase Experiment ! Radioisotopes ! bacteriophage ! DNA Structure ! Hybridization Hybridization Elements are defined by the number of protons they possess Electrons = protons in non-ionized form Radioisotopes Electrons = protons in non-ionized forms #Neutrons can vary leading to different isotopes Additional radioisotopes used in Molecular Biology: P 15 32- important for labeling nucleic acids and phosphorylated proteins mass # atomic # S 16 35- important for labeling proteins Radioactive decay ! Various particles are emitted ( eg. !" particles, #- rays , etc ) ! Several detection methods are available ! geiger counters (emissions in air) ! scintillation counters (radioactive emissions are converted to light) ! autoradiography (X-ray film) autoradiogram Most biological radioisotopes are beta-emitters ! Equivalent to an electron ! Vary in energy ! 3 H- low energy ! 32 P-high energy ! Half-life (t 1/2 )- time at which 1/2 of substance has decayed ! 3 H-12.3 years ! 32 P-14.3 days Viruses that infect bacteria are called bacteriophages ! Viruses are parasites!...
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This note was uploaded on 07/08/2009 for the course LIFESCI LS 3 taught by Professor Paulolague during the Fall '09 term at UCLA.

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LEC02A.PPT [Compatibility Mode] - Last time: ! Griffith...

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