LEC07.PPT [Compatibility Mode]

LEC07.PPT [Compatibility Mode] - 10/10/200 Announcements !...

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10/10/200 8 Announcements Next week Lab 5 ! galactosidase ! Next week – Lab 5- - galactosidase induction ! Quiz 2 " You will have two attempts " Do not use your back button on the browser (it may crash) " Multiple choice or T/F Transcription: synthesis of RNA from a DNA template Prokaryotes
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10/10/200 8 Central Dogma of Molecular Biology Gene expression EM Transcription and translation occur simultaneously in prokaryotes
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10/10/200 8 Overview ! " Template - DNA " Enzyme - RNA polymerase " Steps of transcription Initiation Elongation Termination DNA How does RNA polymerase (P) know where to start? How does RNA P know which strand to use as RNA template? How does RNA P separate the DNA strands? What is the chemical reaction? What happens if it makes a mistake? How does RNA P know where to stop?
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10/10/200 8 RNA Polymerase Bacterial polymerase has 5 subunits ! ! Closely related to all other RNA polymerases (archae, plants and animals)
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10/10/200 8 E. coli RNA Polymerase Core Subunits ! " 2 !! # constitutes the core polymerase " $ # ! Addition of % factor makes holoenzyme i s s ifi DNA s s: % recognizes specific DNA sequences: There are multiple % factors in cell that recognize different sequences.
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10/10/200 8 " ! and ! ’ required for DNA binding and catalysis Functions of the different subunits and required for DNA binding and catalysis " " subunit required for DNA binding and assembly " # subunit stabilizes ! ’ binding " %
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This note was uploaded on 07/08/2009 for the course LIFESCI LS 3 taught by Professor Paulolague during the Fall '09 term at UCLA.

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LEC07.PPT [Compatibility Mode] - 10/10/200 Announcements !...

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