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LEC10 - Announcements Quiz 2 Two attempts Due by Monday...

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Announcements Quiz 2 Two attempts Due by Monday noon • Wednesday Review in class • Midterm 5-7 pm Thursday All material through Monday lecture Central Dogma of Molecular Biology Gene expression
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Gene Expression Transcription: DNA -------> RNA same language (nucleotides), different font ! Translation ! RNA ------> protein ! new language (amino acids), translation required
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Overview mRNA mRNA
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Translation • tRNAs (translators) – charging wobble pairing Ribosomes (protein synthesis machines) rRNA, r-proteins Phases of Translation – Initiation – Elongation – Termination Translation in both prokaryotes and eukaryotes initiates with an AUG which encodes methionine. This can be considered the START of translation, though additional signals are also required. ORF (open reading frame)
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Translation of the genetic code requires an adaptor molecule: -read the code -deliver an amino acid The adaptor is the transfer RNA ( The adaptor is the transfer RNA ( tRNA tRNA) tRNA (transfer RNA) 70 - 80 nucleotides long 30 - 40 different species in bacteria. 50 different species in eukaryotes.
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