LEC11 - Announcements Midterm: 5-7 pm Thursday Broad 2160E...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Announcements • Midterm: 5-7 pm Thursday – Broad 2160E • TAs: Janet, Diana, Ronika, Shawn, Gabriela –Ha ines 39 • TAs: Danny, Rachel, Jessica, Jennifer, Ana Marie – MS4000A • TAs: Lynn, Lauren, Courtney, Allen Translation: in detail
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Ribosomal RNAs • Secondary structure of 16S rRNA contains many stem-loops and can be viewed in 6 domains • 3D structure recently solved by Tom Steitz at Yale for entire large ribosomal subunit (Science, Aug 11, 2000) 30S subunit at 3 o A resolution rRNA and r proteins
Background image of page 2
50S subunit 3D structure Proteins bind to surface and stabilize rRNA folding RNA - gray protein - yellow
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The 70S ribosome (50S+30S) tRNA binding sites on ribosome •A s i t e : (A for aminoacyl tRNA) aminoacyl tRNA will enter at this site •P s i t e : (P for peptidyl-tRNA) new peptide bonds are formed at this site •E s i t e : (E for exit site) transiently occupied by the tRNA that is leaving ribosome
Background image of page 4
In the A and P sites, the tRNAs can make contact with the mRNA Interaction between bound tRNAs and mRNA E PA mRNA tRNA Codon 1 Codon 2 Site of peptide synthesis
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
50S subunit Three important features of protein synthesis • In addition to ribosomes, additional protein factors are required: – Initiation factors (proteins) – Elongation factors –Term inat ion factors •GT P h yd r o l y s i s associated with conformational changes and regulation • ATP provides energy for peptide bond formation • RNAs play a major role in protein synthesis
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 20

LEC11 - Announcements Midterm: 5-7 pm Thursday Broad 2160E...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online