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LEC12 - DNA Replication DNA Replication(S phase Cell...

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DNA Replication DNA Replication (S phase) Cell Cell Division Division (Mitosis) (Mitosis)
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Mechanism proposed by Watson & Crick New strand Old strand Old strand Models for DNA replication (Meselson-Stahl, 1958) Conservative Semi-conservative
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How can one distinguish between the original strands of DNA and the newly synthesized strands? Density label DNA, separate with equilibrium centrifugation on a CsCl gradient
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Experiment: Grow cells in media containing 15 N Shift to media containing 14 N Extract DNA and centrifuge after 1 and 2 generations Predictions Conservative Semi-conservative HH HH H L H L HH LL 1st doubling in 14 N
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HH LL H L H L H L H L LL LL HH LL LL LL 2nd doubling in 14 N
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