LEC20 - 11/16/2008 Announcements Last Quiz this weekend...

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11/16/2008 1 Announcements Last Quiz this weekend th Midterm II: next Thursday, Nov. 20 Broad 2160E: Janet, Diana, Ronika, Shawn, Gabriela, Allen Haines 39: Danny, Rachel, Jessica, Jennifer, Ana Marie MS4000A: Lynn Lauren Courtney MS4000A: Lynn, Lauren, Courtney In class review: Wednesday Friday: no lecture Enhancers contain sequences recognized by transcription factors Fos is a transcription factor that is transcribed in response to many signals Fos is a transcription factor that is transcribed in response to many signals
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11/16/2008 2 Transcription factors are modular proteins DNA binding domain Activation domain Nuclear localization sequence Dimerization domain (some, not all) Ligand-binding domain (some, not all) Nuclear export sequence (some, not all) DNA binding domain Positions transcription factor at particular site by binding to specific particular site by binding to specific sequences Recognizes sequence in double helix Recognition is primarily through hydrogen bond donors and acceptors in major groove
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11/16/2008 3 D: hydrogen bond donor A: hydrogen bond acceptor H: hydrogen not involved in hydrogen bonding M: methyl All four base pairs have unique donors and acceptors in the major groove In the minor groove cannot distinguish AT vs TA or GC vs CG
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11/16/2008 4 Alpha helix fits perfectly into major groove allowing H-bonding with donors and acceptors: in this way it can read the DNA sequence Amino acid side chains make specific hydrogen bonds with groups on bases exposed to major groove
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11/16/2008 5 Dimeric protein reading sequence
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This note was uploaded on 07/08/2009 for the course LIFESCI LS 3 taught by Professor Paulolague during the Fall '09 term at UCLA.

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LEC20 - 11/16/2008 Announcements Last Quiz this weekend...

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