LEC21 - 11/18/2008 1 Announcements • Midterm II: next...

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Unformatted text preview: 11/18/2008 1 Announcements • Midterm II: next Thursday, Nov. 20 th – Broad 2160E: Janet, Diana, Ronika, Shawn, Gabriela, Allen – Haines 39: Danny, Rachel, Jessica, Jennifer, Ana Marie – MS4000A: Lynn, Lauren, Courtney • In class review: Wednesday • Friday: no lecture AcK- enriched in euchromatin Bromo domain- binds AcK Chromotin remodelers recruited to euchromatin Open chromatin further Chromatin remodeling protein Open chromatin further 11/18/2008 2 Chromatin remodeling complexes • In addition to modifying nucleosomes directly, chromatin remodeling complexes use ATP to: – Slide nucleosomes – Remodel nucleosomes – Dissociate histones from DNA – Replace nucleosomes Chromatin remodelers 11/18/2008 3 Gel shift assay detects change in migration of nucleosome At some promoters, chromatin remodelers and HATs are recruited before RNA Pol II Chromatin remodeler Histone acetyltransferase Sequence specific TF Sequence specific TF 11/18/2008 4 At others, RNA Pol II is at the promoter but can not initiate until remodeling occurs Histone acetyltransferase Chromatin remodeler Preinitiation complex TAF250- HAT activity How do these changes in chromatin fit in with transcription? • In the 1990s it was found that a number of transcription factors identified genetically or biochemically were in fact chromatin modifiers • Intensive studies mapping modifications, and following changes upon induction of promoters, have yielded some insight into how chromatin is modified during transcription 11/18/2008...
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This note was uploaded on 07/08/2009 for the course LIFESCI LS 3 taught by Professor Paulolague during the Fall '09 term at UCLA.

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LEC21 - 11/18/2008 1 Announcements • Midterm II: next...

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