LS420lec2final28322012920DOC

LS420lec2final28322012920DOC - LS4 Lec2 Final Exam 3/22/01...

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1 LS4 Lec2 Final Exam 3/22/01 ___________________________ ______________________ ___________________ Last name First SID Disc. Section 1 ______________ 5 ______________ 9 ______________ 13 _____________ 2 ______________ 6 ______________ 10 _____________ 14 _____________ 3 ______________ 7 ______________ 11 _____________ 15 _____________ 4 ______________ 8 ______________ 12 _____________ 16 _____________ Total __________________ Discussion __________________ Midterm 1 __________________ Midterm 2 __________________ Quarter total score __________________ Quarter grade __________________ Show All Your Work for Full Credit.
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2 1. (6 points) The pedigree below shows inheritance of Tay-Sachs disease, an autosomal recessive trait, in two unrelated families. The indicated couple (II.2 and II.3) would like to have children. What is the probability that, if the couple has 3 children, none of them will be affected by Tay-Sachs disease? 2. (6 points) Brown mice with strong white spots crossed to solid black mice give all brown progeny with weak white spots. Brother-sister crosses among the F1 generate these F2: Brown with weak white spots 24 Brown with strong white spots 12 Solid brown ( no spots ) 12 Black with weak white spots 8 Black with strong white spots 4 Solid black ( no spots ) 4 Circle all of the following genetic phenomena that are needed to explain these results. incomplete dominance lethal alleles epistasis multiple genes multiple alleles duplicate genes
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3 3. (9 points) A circular plasmid DNA was digested with BamH1, Sal1 or both and the restriction fragments separated by gel electrophoresis. After staining the DNA, the following pattern is observed (the numbers indicate the size of the restriction fragments in kilobases): a. Determine a restriction map of the plasmid from the data above. b. If the DNA in the gel above was transferred to a nitrocellulose paper and probed with the 3.6 kb fragment, which bands would show up on the autoradiogram?
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4 4. (12 points) a. The diagrams below are for an animal species with chromosome number 2N = 4. (1) For each diagram, indicate what stage in mitosis or meiosis the cell is in. (2) What aspects of the diagram allowed you to make this distinction? Describe them.
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LS420lec2final28322012920DOC - LS4 Lec2 Final Exam 3/22/01...

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