Answers%20to%20March%204%20questions

Answers%20to%20March%204%20questions - Answers to March 4...

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Answers to March 4 questions: 1. amplification of the DNA to use for chemical analysis, get specific sequences such as specific genes 2. PCR will do amplification if you know the sequences in the DNA to use for primers 3. cloning, since you don’t start with knowledge of the leu+ sequence 4. it can be done with any vector if the detection system is an oligonucleotide probe dictated by the amino acid sequence of beta globin. It can be an expression vector if an antibody against beta globin is available to detect the clone producing beta globin peptide. 5. A large collection of bacteria (or yeast cells) each with a DNA clone from a population of different clones. 6. Red blood cell mRNA 7. cDNA is relatively compact, which is good, although the genomic beta globin sequence turns out to be compact with 3 exons and 2 small introns. Most eukaryotic genomic sequences have quite large transcription domains with many exons and introns and are not readily clonable, at least not in their entirety. 8. Breaking a larger clone, such as a BAC, into many smaller (1kb) fragments to clone into a vector with sequencing primer ends. Computers are used to create the reported sequence.
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This note was uploaded on 07/08/2009 for the course LIFESCI ls 4 taught by Professor Merriam during the Winter '08 term at UCLA.

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Answers%20to%20March%204%20questions - Answers to March 4...

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