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forensic DNA Monday may 12

Most minisatellites occur at multiple sites around

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Unformatted text preview: ccur at multiple sites around the genome. DNA fingerprint pattern of simultaneous genotypes at a group of unlinked loci Use restriction enzymes and southern blots to detect length differences at minisatellite loci. Most useful minisatellites have 10 20 sites around genome and can be analyzed on one gel. Minisatellite analysis by Southern blots Question: who could be related among A, B, C & D in this diagram? Answer: Fig. 11.14 DNA fingerprints can identify individuals and determine parentage. e.g., DNA fingerprints confirmed Dolly the sheep was cloned from an adult udder cell. Donor udder (U), cell culture from udder (C), Dolly's blood cell DNA (D), and control sheep 1-12 Problems are 1. Not quantitative, and 2. Requires large amount of DNA Fig. 11.15 Forensic use, replace with Microsatellites, also called sequence (simple) tandem reapeat polymorphisms = STRPs 1 every 30,000 bp Repeated units 2 5 bp in length Mutate by replication error Mutation rate of 10-3 per locus per gamete Useful as highly polymorphic DNA markers Fig. 11.3 Microsatellite allele detection analysis of size differences by PCR can distinguish genotypes Fig. 11.12 Fig. 9.16.1 Fig. 9.16.2 Fig. 9.16.3 Fig. 9.16.4...
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