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Unformatted text preview: utions Arise by mutagenic chemicals or mistakes in replication Biallelic only two alleles Ratio of alleles ranges from 1:100 to 50:50. 2001 over 5 million human SNPs identified Most occur at anonymous loci. Mutation rate of 1 X 10-9 per locus per generation Very few are thus new mutation in the species. Useful as DNA markers Fig. 10.21 Microchips place single strand DNA oligomers (many copies) on chip. Vary sequence by 1 nt at different positions on chip. Hybridize (at high stringency) with labeled single strand DNA probe from person to detect which oligos are bound. Fig. 10.22 Compare 2 samples, differently labelled, simultaneously as control for hybridization efficiency. Black is no hybridization, yellow is both probes hybridize. Fig. 10.20 Alternative is high speed sequencing by Sanger method. Fig. 9.18 Fig. 13.3 nucleosome structure characterizes eukaryotic DNA-How packaged? Why follow DNA heredity?...
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This note was uploaded on 07/08/2009 for the course LIFESCI ls 4 taught by Professor Merriam during the Winter '08 term at UCLA.
- Winter '08