HIV functional genomic screen

HIV functional genomic screen - DOI:...

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Unformatted text preview: DOI: 10.1126/science.1152725 , 921 (2008); 319 Science et al. Abraham L. Brass, Infection Through a Functional Genomic Screen Identification of Host Proteins Required for HIV www.sciencemag.org (this information is current as of June 1, 2008 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/319/5865/921 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1152725/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.sciencemag.org/cgi/content/full/319/5865/921#related-content http://www.sciencemag.org/cgi/content/full/319/5865/921#otherarticles , 15 of which can be accessed for free: cites 35 articles This article 4 article(s) on the ISI Web of Science. cited by This article has been http://www.sciencemag.org/cgi/content/full/319/5865/921#otherarticles 1 articles hosted by HighWire Press; see: cited by This article has been http://www.sciencemag.org/cgi/collection/medicine Medicine, Diseases : subject collections This article appears in the following http://www.sciencemag.org/about/permissions.dtl in whole or in part can be found at: this article permission to reproduce of this article or about obtaining reprints Information about obtaining registered trademark of AAAS. is a Science 2008 by the American Association for the Advancement of Science; all rights reserved. The title Copyright American Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. (print ISSN 0036-8075; online ISSN 1095-9203) is published weekly, except the last week in December, by the Science on June 1, 2008 www.sciencemag.org Downloaded from Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen Abraham L. Brass, 1,2 Derek M. Dykxhoorn, 3 * Yair Benita, 4 * Nan Yan, 3 Alan Engelman, 5 Ramnik J. Xavier, 2,4 Judy Lieberman, 3 Stephen J. Elledge 1 † HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediator complex (Med28) in viral transcription. Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally perform the functions required for their life cycle. This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing...
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HIV functional genomic screen - DOI:...

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