Lecture 11 - Conjugation

Lecture 11 - Conjugation - Lecture 11 Conjugation Joshua...

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Lecture 11 – Conjugation Joshua Lederberg joined Edward Tatum’s laboratory with the intention of discovering genetic recombination in bacteria! …and early attempts worked! “In 1941… the question whether ‘bacteria have genes, like all other organisms’ was still unanswered, indeed rarely asked… “It took about six weeks, from the first serious efforts at crossing in mid-April 1946, to establish well-controlled, positive results [for a genetic cross].” –– Fortieth year anniversary reminiscence, J. Lederberg
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Genetic Markers Auxotroph Genotype: thr + = threonine-biosynthesis gene = a defective threonine-biosynthesis gene Phenotype: Thr + = Growth on medium lacking threonine Thr = No growth on medium lacking threonine master plate replica plate 2 replica plate 1 MM + threonine Minimal Medium (MM) rich medium
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Genetic Markers Carbon Source Genotype: lacZ + = beta -galactosidase gene = defective -galactosidase gene Phenotype: Lac + = Growth on medium containing lactose as carbon source
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This note was uploaded on 07/08/2009 for the course LIFESCI ls 4 taught by Professor Merriam during the Winter '08 term at UCLA.

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Lecture 11 - Conjugation - Lecture 11 Conjugation Joshua...

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