whatisagenechap7wedmay7

whatisagenechap7wedmay7 - May 7, 2008 What is a gene?...

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May 7, 2008 What is a gene? Anatomy and Function of a Gene Dissection through Mutation
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What mutations tell us about gene structure Gene is at a place on the chromosome (locus). Gene codes for a protein (enzymatic activity). Complementation testing tells us whether two mutations are in the same or different genes.
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complementation groups (different genes) for eye color. Recombination mapping demonstrates distance between genes and alleles. Fig. 7.18 b,c
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Extend recombination mapping from between genes into gene. What mutations tell us about gene structure. Seymore Benzer mid 1950s – 1960s If a gene is a linear set of nucleotides, recombination between homologous chromosomes carrying different mutations within the same gene should generate wild-type. T4 phage as an experimental system: Can examine a large number of progeny to detect rare mutation events Could allow only recombinant phage to proliferate while parental phages die
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How recombination within a gene could generate wild-type Fig. 7.19
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Working with T4 phage to see rare events Fig. 7.20 a
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Fig. 7.20.b.1 Phage plaque mutant phenotypes
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Fig. 7.20.b.2 Conditional growth (reproduction) permissive vs restrictive
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Fig. 7.20.c.1 Complementation test for mutations in same or different genes Restrictive condition
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whatisagenechap7wedmay7 - May 7, 2008 What is a gene?...

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