MIMG 106 lecture staph aureaus

MIMG 106 lecture staph aureaus - Staphylococcus aureus...

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Staphylococcus aureus Virulence Gene Regulation, Superantigens, and Antibiotic Resistance Ken Bradley, Ph.D. Asst. Professor Microbiology, Immunology & Molecular Genetics Tel: 310-206-7465 Office: MSB 2825A E-mail: kbradley@microbio.ucla.edu
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Staphylococcus aureus • Gram positive facultative anaerobe Staphyle - Greek for “grape cluster” aureus - Latin for “gold”
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Lecture Overview 1) Introduction to virulence factors 2) Virulence gene regulation 3) Surface Proteins 4) Exotoxins and Superantigens 5) Antibiotic Resistance
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Where does S. aureus come from? Found on most people at some time during life – 1/3 of human population is colonized at any point in time – 25% colonized persistently stye furuncles boils, carbuncles pneumonia emesis UTI cystitis hematogenous spread sinusitis osteomyelitis SSS TSS diarrhea impetigo endocarditis Adapted from http://www.textbookofbacteriology.net/
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1) S. aureus Virulence Factors Adapted from http://www.textbookofbacteriology.net/
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2) Virulence Gene Expression • STM methods only identified genes required for growth (housekeeping) - thus “virulome” must be tested one protein at a time. • Agr quorom sensing - regulates virulence gene expression – Up regulation of secreted proteins and downregulation of surface proteins
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Accessory Gene Regulation(Agr)
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Agr Regulation - AIP • Auto-inducing peptide (AIP) derived from Agr D • Agr system conserved in staphylococci. • Structure = 7-9 amino acid thiolactone peptides. Novick, R. 2003 Mol. Micro 48(6)
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Accessory Gene Regulation(Agr)
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Accessory Gene Regulation(Agr)
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Agr Effector is RNA III Possible Mechanisms : C-rich stem loops contain SD- complementary sequences (Novick, 2003) Translation-activation of hla (Morfeldt, 1995) Translation-repression of spa . (Huntzinger, 2005) Antisense RNA and targeting for RNase-III mediated degradation (Boisset, 2007)
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agr agr ABCD BCD ( I) I) S. aureus AIP (II) AIP (I) Lyon et al., 2000 PNAS v97 agr agr ABCD BCD ( II) II) S. aureus
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This note was uploaded on 07/11/2009 for the course MIMG 106 taught by Professor Bradley,shi during the Spring '09 term at UCLA.

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MIMG 106 lecture staph aureaus - Staphylococcus aureus...

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