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sp04-2nd%20exam - 1 Metabolic Biochemistry / BIBC 102...

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Unformatted text preview: 1 Metabolic Biochemistry / BIBC 102 Second Exam / Spring 2004 I. (20 points) Fill in all of the enzyme catalyzed reactions which convert the substrate on the upper left to the product on the lower right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, Pi, NAD + , FAD, NADH, etc.), any required cofactors (e.g., biotin, TPP, etc.), and indicate subcellular location (cytosol or matrix). O A. CH 3 (CH 2 ) 14 C-O (Cytosol) O O CH 3 (CH 2 ) 12 C SCoA + CH 3 C SCoA (matrix) (matrix) B. Please indicate which one of the above enzymatic reactions is under allosteric regulation , the identity of the allosteric activators and/or inhibitors of this enzyme, and the physiological role of this regulation in a cell. 2 II. (20 points) (a) Draw a diagram of the overview of proton and electron circuits in mitochondria starting with NADH in the matrix and ADP and P i in the cytosol and ending with the reduction of oxygen to water and the formation of ATP (the diagram in the lecture handout will receive full credit). Label each complex and protein, identify matrix, intermembrane space, and inner membrane, and indicate the flow of protons and electrons. (b) Draw, on this diagram, the entire glycerophosphate shuttle , with the correct structure of each intermediate molecule and the use of NAD, FAD, TPP, biotin, carnitine, or any other required molecule in the glycerophosphate shuttle reactions. 3 III. (20 points) Fill in all of the enzyme catalyzed reactions which convert the substrate on the upper left to the product on the lower right. Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, Pi, NAD + , FAD, NADH, etc.), any required cofactors (e.g., biotin, TPP, etc.), and indicate the subcellular location (cytosol or matrix)!!! A. -hydroxybutyrate (draw) A c e t y l C o A ( d r a w )----------------------------------------------------------------------------------------------------------------------------- B....
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This note was uploaded on 07/13/2009 for the course BIBC BIBC 102 taught by Professor Price during the Spring '08 term at UCSD.

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sp04-2nd%20exam - 1 Metabolic Biochemistry / BIBC 102...

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