BISC 220 Lab 5 expected results - Chapter 5 Cellular Respiration and Fermentation Specifications Mitochondrial preparation from liver 1 Blend 15 g liver

Chapter 5: Cellular Respiration and Fermentation Specifications Mitochondrial preparation from liver 1. Blend 15 g liver with 35 mL of cold grinding solution (.5M sucrose , +.1M potassium phosphate, pH 7.3) 2. Filter suspension and centrifuge filtrate at 500 x g for 10 minutes at +4’C 3. Decant supernatant and centrifuge for 30 minutes at 5000 x g and 4’C 4. Discard supernatant, suspend sediment in 10 mL suspension solution (.4M +sucrose, .02M potassium phosphate, .005 M magnesium chloride and .02 M glucose. 5. Freeze or use immediately. Activity 1: Alcoholic Fermentation Table 2 Tube# Tube 1 Tube 2 Tube 3 Tube 4 Rate of fermentation 0 0 slower than Tube 4 faster than Tube 3 Activity 2: Cellular Respiration Warning Another expect result (case 2) is added. Note this has nothing to do with the “alternative hypothesis” in your lab report . Both cases have their own null hypothesis and alternative hypothesis. Tough it can serve as the “alternative explanation” you might use in the discussion part. -yichun Table 4 (Absorption) Depending on how many working mitochondria was in the chicken liver that was used, probably related to how fresh the chicken liver was. The amount of succinate can be either in excess or not, giving two difference cases: Case 1 If the succinate in Tube 2 and Tube 3 are not in excess, the capacity of mitochondria is not reached: Tube# Tube 1 Tube 2 Tube 3 Trend of absorption reading nearly constant decreasing slower than tube 3 descreasing faster than Tube 2 Case 2