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Unformatted text preview: Chapter 1: Biological Materials and Enzyme Action Part A 1. Test for carbohydrates A. Test for starch Test with IKI, or Lugols iodine. 1 Potato scrapings in water + 2 Apple srapings in water + 3 Egg white- 4 Cracker crumbs in water + B. Test for simple sugars Benedicts test. 1 Chopped liver- 2 Potato scrapings + 3 Apple scrapings + 4 Whole milk + 2. Test for lipids Test with Sudan IV. Note The procedure of Sudan test changed after Tuesday. I will get the new procedure and write it down here. 1 Whole milk + 2 Chopped liver- 3 Potato scrapings- 4 Egg yolk + 3. Test for proteins See part B Part B 1. Action of Pancreatic Enzymes on Starch Sample Result Tube A (pancreatin, starch and IKI)- Tube B (boiled pancreatin, starch and IKI) + Tube C (pancreatin and IKI) control- Tube D (starch and IKI) control + Note Many pointed it out to me that in Tube B, the dark-blue color disappeared after mixed well. I am still thinking about why it was so. If you have an idea please discuss it with me. Update: the explanation could be, you did not boil tube B heavy enough so all the pancreatin was not denatured.Update: the explanation could be, you did not boil tube B heavy enough so all the pancreatin was not denatured....
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- Spring '09