12_TestBank_TB - Biology: Concepts and Connections, 5e...

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Biology: Concepts and Connections, 5e (Campbell) Chapter 12: DNA Technology and the Human Genome 1) When DNA fingerprinting was first used, A) genetic evidence was collected using only DNA from blood. B) blood samples from the crime scene were used to match the blood of a person who confessed. C) the identity of two semen samples did not match to a person who confessed. D) genetic testing revealed that the two murdered girls were killed by two different murderers. E) None of the choices are correct. Topic: Introduction Skill: Factual Recall 2) When plasmids are used to produce a desired protein A) the plasmids are inserted into the bacterial chromosome. B) the plasmids multiply and produce the protein outside of the bacterium. C) the bacterial chromosome is genetically engineered and the plasmid is used to help the bacterium replicate. D) the desired gene is inserted into the plasmid and the plasmid is returned to the bacterium by transformation. E) the bacterial genome and plasmid are inserted into the genome of the cell containing the desired gene (perhaps the cell of a plant or animal). Topic: 12.1 Skill: Factual Recall 3) The production of multiple identical copies of gene-sized pieces of DNA defines A) gene cloning. B) plasmid transformation. C) clonal selection. D) tissue culturing. E) plasmolysis. Topic: 12.1 Skill: Factual Recall 4) Restriction enzymes A) edit proteins. B) cut DNA at specific sites. C) stop transcription. D) bind together strands of DNA. E) bind RNA fragments together. Topic: 12.2 Skill: Factual Recall
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5) "Sticky ends" are A) produced by the action of DNA ligase. B) produced by PCR. C) always long sequences of a single nucleotide. D) used by mRNA to attach to ribosomes. E) DNA fragments with single-stranded ends. Topic: 12.2 Skill: Factual Recall 6) DNA ligase binds A) exons together. B) polymerase to the promotor. C) nucleotides together. D) introns together. E) All of the choices are correct. Topic: 12.2 Skill: Factual Recall 7) The feature of "sticky ends" that makes them especially useful in DNA recombination is their ability to A) bind to DNA and thereby activate transcription. B) bind to ribosomes and thereby activate translation. C) form hydrogen-bonded base pairs with complementary single-stranded stretches of DNA. D) allow plasmids to attach to the main bacterial chromosome. E) insert a segment of RNA into a bacterial chromosome. Topic: 12.2 Skill: Conceptual Understanding 8) In the process of human gene cloning using recombinant plasmids, the bacterial plasmid A) functions as a vector. B) is the source of the gene to be cloned. C) is cultured inside the human cell which contains the gene to be cloned. D) is used to insert the human gene into the bacterial chromosome. E) None of the choices are correct.
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This note was uploaded on 07/16/2009 for the course BSC BSC1005 taught by Professor Magellantracy during the Spring '09 term at Miami Dade College, Miami.

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12_TestBank_TB - Biology: Concepts and Connections, 5e...

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