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BIO 191 15.3 - 15.3 Monday 11:13 PM Transcription in...

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15.3 Monday, November 28, 2005 11:13 PM Transcription in Prokaryotes RNA Polymerase Very Large and Complex consists of 5 subunits 1. Two alpha subunits- Regulatory Proteins 1. Beta subunit- Ribonucleotide 1. Beta Prime subunit- DNA template 1. Sigma subunit recognizes the promoter and initiates synthesis 2 Strands of DNA Template Strand Strand that is transcribed Coding Strand Strand that is not transcribed Has same sequence as the RNA transcript, except T takes the  place of U Adds ribonucleotides to the growing 3' end of an RNA chain. RNA Polymerase II binds extremely efficiently to consensus. Has no proofreading ability. Promoter Where transcription starts On DNA template strand A short sequence that is not itself transcribed by the polymerase that binds to   it. -35 sequence located 35 nucleotides upstream of the position where   transcription actually starts
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