L4 Outline - BIO 320 - CELL BIOLOGY Summer 2009 A. De...

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BIO 320 - CELL BIOLOGY – Summer 2009 A. De Lozanne Lecture 4: Sorting of Proteins into Organelles Membrane Trafficking: Overview of Secretory Pathway, Signal Peptide Hypothesis, Stop-transfer Sequences and the Endoplasmic Reticulum. Reading: Alberts, 5th Ed. Pages 695-704 and 723-736 When you have finished this lecture and the assigned reading, you should be able to:     1. Explain the structural and functional relationship between the endoplasmic reticulum  (rough and smooth) , Golgi complexes, lysosomes, endosomes, vacuoles and plasma  membranes of eukaryotic cells. a. Describe the pathway of proteins destined for secretion, starting with synthesis and  ending with exocytosis (secretion).  Describe the pathway of membrane proteins. b. Point out the differences between the syntheses of secretory/integral membrane  proteins and cytosolic proteins.   c.  Explain the difference between “bound” and “free” ribosomes.  Which ribosomes  are used for different kinds of proteins? 2. Summarize the mechanisms whereby newly synthesized proteins are targeted and sorted  for their appropriate destinations. 3.  Understand how sorting signals are used to traffic proteins.   4.  Understand the how the signal peptides, SRP and the SRP receptor direct nascent mRNAs  for secretory proteins to the ER. 5. Describe and understand how the combination and placement of signal peptides, the  charges around the signal peptide, the presence of cleavage site(s) for signal peptidase, and  stop-transfer sequences in a polypeptide chain can determine the orientation of 5 kinds of  polypeptides in the secretory path.  Describe how these sequences can determine whether a  protein is:  (a) a secretory protein (b) an integral membrane protein with its amino-terminus  on the cytosolic side of the membrane (c) an integral membrane protein with its carboxyl- terminus on the cytosolic side of the membrane (d) an integral membrane protein that spans  the membrane several times with its carboxyl-terminus on the cytosolic side of the membrane  (e) an integral membrane protein that spans the membrane several times with its carboxyl- terminus on the cytosolic side of the membrane Lecture Outline
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BIO 320 - CELL BIOLOGY – Summer 2009 A. De Lozanne Outline I. General Aspects of Protein Sorting A. Proteins encoded by nuclear genes may have localization signals. B. The localization signal is a specific peptide sequence within the sequence of the entire 
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This note was uploaded on 07/22/2009 for the course BIO 89545 taught by Professor De lozanne during the Spring '09 term at University of Texas at Austin.

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L4 Outline - BIO 320 - CELL BIOLOGY Summer 2009 A. De...

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