Test2-2008answers - BIO-320 Name _ Cell Biology Spring 2008...

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BIO-320 Name _____________________________________ Cell Biology – Spring 2008 --------------------------------------------------------------------------------------------------------------------- PERSONAL EID __________________ Multiple choice________ Fill in + Essays________ TOTAL SCORE ________ Page 1 of 10 SECOND MIDTERM EXAM (04/01/2008) Before you do anything else, please: Check to make certain that your copy of the exam is complete, i.e. pages 1 - 10. Print your name legibly in the space provided at the top of each page, and also on the scantron bubble sheet. BE SURE TO FILL IN YOUR EID IN THE CORRESPONDING SPACES IN THE SHEET AND ON THIS PAGE. Total possible points = 100 points. The essays MUST be written in pen. If you use a pencil you cannot request a regrade. MULTIPLE CHOICE [2.5 points/question] Answer the 26 multiple-choice questions by marking the appropriate letter under the same number on your scantron bubble sheet. 1) When comparing nuclear transport and mitochondrial transport, which of the following statements is TRUE? a) Both use a signal peptidase. b) Both have a localization signal at their carboxyl terminus. c) Nuclear import requires ATP while mitochondrial import requires GTP. d) Both occur post-translationally. e) Both need the activity of HSP-70. 2) Where in the cell do you find the highest concentration of GDP-Ran and why? a) In the nucleus because that is the location of RanGEF b) In the nucleus because that is the location of RanGAP c) In the nuclear pore complex because the energy of GTP is used for transport across the pore complex. d) In the cytoplasm because that is the location of RanGEF e) In the cytoplasm because that is the location of RanGAP 3) Which of the following statements about the nuclear pore complex (NPC) is FALSE? a) The NPC is the only route for entry of proteins into the nucleus. b) Nucleoporins regulate the formation of Nuclear Import Complexes. c) The NPC is a very large multiprotein complex composed of nucleoporins, Ran and import receptors. d) Some nucleoporins form a hydrogel that forms a barrier for large proteins across the pore of the NPC. e) The NPC allows the free diffusion of ions into and out of the nucleus. 4) Which of the following statements is TRUE in regard to the protein PORIN? a) Porin is used to transport proteins into the mitochondria. b) Porin allows the diffusion of small molecules into the matrix of the mitochondria. c) Porin is found in the outer membrane of the mitochondria. d) Porin is a eukaryotic protein not found in prokaryotes. e) Porin binds to the mitochondrial targeting signal of proteins.
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BIO-320 Cell Biology – Spring 2008 Name _____________________________________ Page 2 of 10 5) An important difference between nuclear import and nuclear export is that: a) The nuclear import complex is only formed in the absence of RanGTP while the nuclear export complex is only formed in the presence of RanGTP b) The nuclear import complex is only formed in the absence of RanGDP while the
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Test2-2008answers - BIO-320 Name _ Cell Biology Spring 2008...

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