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Ch_322a_2.016 - Spectrophotometric Analysis Beer's Law...

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Spectrophotometric Analysis: Beer's Law Diagram of a Dual Beam Spectrometer source of ir radiation I o I o I o reference cell sample cell I o ' I wavelength selector I o ' ( ! ) I ( ! ) photometer recorder or computer The intensities of the transmitted radiation I o ' and I are compared at different ! . When I o ' = I , there is no net absorption by the sample. 100% transmission.
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Beer's Law The absorption of radiation by materials is described by the empirical Beer's Law or Beer-Lambert Law. sample conc., C I o ' ( ! ) I ( ! ) incident light transmitted light path length, b The intensity of the transmitted radiation at a specific wavelength is given by I ( ! ) = I o ' ( ! ) 10 - a ! bc a ! is the intrinsic absorptivity of the material at ! b is the path length of cell or sample in cm c is the concentration in M
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Spectral Presentations of Beer's Law Data Beer's Law relates the amount of radiation absorbed to the concentration of the material. The data may be presented by either the Absorbance (A) or Transmission T or %T at a particular wavelength.
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