PCR 특집 Series 8 TaKaRa PCR Enzymes

PCR - TaKaRa TaqTM TaKaRa Ex TaqTM PyrobestTM DNA Polymerase TaKaRa LA TaqTM TaKaRa LA TaqTM with GC Buffer Premix TaqTM(TaKaRa TaqTM Version

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Unformatted text preview: TaKaRa TaqTM TaKaRa Ex TaqTM PyrobestTM DNA Polymerase TaKaRa LA TaqTM TaKaRa LA TaqTM with GC Buffer Premix TaqTM (TaKaRa TaqTM Version) Premix TaqTM (TaKaRa Ex TaqTM Version) PerfectShotTM Ex Taq ( : TaKaRa Ex TaqTM) ( One Shot LA PCRTM Mix : TaKaRa LA TaqTM) Life Science & Biotechnology No.8 - 2 - TaKaRa TaqTM/ TaKaRa Ex TaqTM/ TaKaRa LA TaqTM/ PyrobestTM DNA Polymerase Life Science & Biotechnology No.8 - 3 - Life Science & Biotechnology No.8 - 4 - Life Science & Biotechnology No.8 - 5 - 176,000 (250 U) 120,000 198,000 (250 U) 170,000 Life Science & Biotechnology No.8 - 6 - Life Science & Biotechnology No.8 - 7 - Life Science & Biotechnology No.8 - 8 - Life Science & Biotechnology No.8 - 9 - Life Science & Biotechnology No.8 - 10 - Target cell : MKN-45 (Treated with IFN- (100 U/ml) for 48 hrs) Effector to Target=3 anti-class I mAb: W6/32 anti-class II mAb: L243 anti-CD3 mAb: OKT-3 % Lysis Effector/Target MKN1 and MKN45 were treated with IFN- (100 U/ml) for 48 hrs Life Science & Biotechnology No.8 - 11 - % Lysis Effector/Target Life Science & Biotechnology No.8 - 12 - 98 TaKaRa International Symposium Organizer & Chair : Young Min Kim, Ph.D./Yonsei Univ. Je Hyeon Lee, Ph.D./BOHAN Biomedical Inc. 1. Recent progress of genome sequencing projects Seung-Whan Park Ph.D./KRIBB, KIST, Korea 2. DNA array chip: New generation for functional genomics Ikunoshin Kato Ph.D./TAKARA Biomedical Reaserch Labs., Japan 3. From sequencing genomics to functional genomics: The role of bioinformatics Seung-Moak Kim Ph.D./KRIBB, KIST, Korea 4. Advanced Technology of Polymerase Chain Reaction(PCR): Development of new enzyme for rapid PCR technology Kiyozo Asada Ph.D./ TAKARA Biomedical Reaserch Labs., Japan TAKARA-KOREA BIOMEDICAL INC. Life Science & Biotechnology No.8 - 13 - Life Science & Biotechnology No.8 - 14 - Life Science & Biotechnology No.8 - 15 - Life Science & Biotechnology No.8 - 16 - Life Science & Biotechnology No.8 - 17 - 1) Kinoshita, T., Imamura, J., Nagai, H., Shimotohno K. : Anal. Biochem. 206, 231-235(1992) 2) Nakayama, H., Yokoi, H., Fujita, J. : Nucl. Acids Res., 20, 4939(1992) 3) Dicesare, J., Grossman, B., Katz, E., Picozza, E., Ragusa, R., Woudenberg. T. : Biotechniques, 15, 152-157(1993) 4) Chelly, J., Kaplan, J. C., Maire, P., Gautron, S., Kahn, A. : Nature, 333, 858-860(1988) 5) Noonan, D. E., Beck, C., Holzmayer, T. A., Chin, J. E., Wunder, J. S., Andrulis, I. L., Gazdar, A. F., Willman, C. L., Griffith, B., VonHoff, D. D., Roninson, I. B. : Proc. Natl. Acad. Sci. USA, 87, 71607464(1990) 6) Murphy, L. D., Herzog, C. E., Rudick, J. B., Fojo, A. T., Bates, S. E. : Biochemistry, 29, 10351-10356(1990) 7) Horikoshi, T., Danenberg, K. D., Stadlbauer, T. W., Volkenandt, M., Shea, L. C., Aigner, K., Gustavsson, B., Leichman, L., Frsing, R., Ray, M., Gibson, N. W., Spears, C. P., Danenberg, P. V. : Cancer Res., 52, 108-116(1992) 8) Yokoi, H., Natsyama, S., Iwai, M., Noda, Y., Mori, T., Mori, K., Fujita, K., Nakayama, H., Fujita, J. : Biochem. Biophys Res. Commun., 195, 769-775(1993) 9) Becker-Andre, M., Hahlbroc, K. : Nucl. Acids Res., 17, 94379446(1989) 10) Gilliland, G., Perrin, S., Blanchard, K., Bunn, H. F. : Proc. Natl. Acad. Sci. USA. 87, 2725-2729(1990) 11) Diviacco, S., Norio, P., Zentilin, L., Menzo, S., Clementi, M., Biamonti, G., Riva, S., Falaschi, A., Giacca, M. : Gene, 122, 313320(1992) 12) Celi, F. S., Zenilman, M. E., Shuldiner, A. R. : Nucl. Acids Res., 21, 1047(1993) 13) Vanden Heuvel, J. P., Tyson, F. L., Bell, D. A. : Biotechniques, 14, 395-398(1993) 14) Siebert, P. D., Larrick, J. W. : Nature, 359, 557-558(1992) 15) Siebert, P. D., Larrick, J. W. : Biotechniques, 14, 244-249(1993) 16) Hayashi : , 29, 513-517(1992) Life Science & Biotechnology No.8 - 18 - Life Science & Biotechnology No.8 - 19 - Life Science & Biotechnology No.8 - 20 - Life Science & Biotechnology No.8 - 21 - Life Science & Biotechnology No.8 - 22 - Long Ranger SingelTM Pack Q & A Q1 A1 Q2 A2 Life Science & Biotechnology No.8 - 23 - CTL Epitope Peptide Set MAGE3(HLA-A24), CEA(HLA-A24), HER2/neu(HLA-A24) Life Science & Biotechnology No.8 - 24 - Life Science & Biotechnology No.8 - 25 - Kit LDH Cytotoxicity Detection Kit Life Science & Biotechnology No.8 - 26 - Life Science & Biotechnology No.8 - 27 - absorbance(A492nm-A690nm) Cells/well absorbance(A492nm-A690nm) detergent conc. (%) Life Science & Biotechnology No.8 - 28 - killer : target cell ratio cell mediated cytotoxicity (%) absorbance(A492nm-A690nm) killer : target cell ratio (X105) days in culture Life Science & Biotechnology No.8 - 29 - absorbance(A492nm-A690nm) cells/ml ApoPrimer Set(Bcl-2 family) Life Science & Biotechnology No.8 - 30 - bak-S bik-S mcl-1-S bcl-x-S bax- -S bcl-2-S bfl-1-S -actin-S bak-A bik-A bcl-x-A bax- -A -actin-A bcl-2-A bfl-1-A mcl-1-A poly (A) Life Science & Biotechnology No.8 - 31 - Label ITTM Life Science & Biotechnology No.8 - 32 - Q & A Q1 A1 Q3 A3 Q2 A2 Life Science & Biotechnology No.8 - 33 - mRNA Selective PCR Kit Ver. 1.1 Life Science & Biotechnology No.8 - 34 - Life Science & Biotechnology No.8 - 35 - Non-RI Labeling BcaBESTTM DIG Labeling Kit Life Science & Biotechnology No.8 - 36 - Universal Tyrosine Phosphatase Assay Kit Life Science & Biotechnology No.8 - 37 - r = 0.996 log Y = 0.143-0.783logX r = 1.000 log Y = 0.416-83.05X Life Science & Biotechnology No.8 - 38 - Q & A Q1 A1 Q5 A5 Q6 A6 Q7 Q2 A2 A7 Q8 Q3 A3 A8 Q4 A4 Life Science & Biotechnology No.8 - 39 - Transferrin polyethylenimine DNA complex Transferrin polyethylenimine DNA complex Transferrin receptor Transferrin receptor CYTOPLASMA CYTOPLASMA ENDOSOME pH drops Life Science & Biotechnology No.8 - 40 - Reliant RNA Gel System 1.25% SeaKem Gold Agarose MutationFinderTM Kit Life Science & Biotechnology No.8 - 41 - Life Science & Biotechnology No.8 - 42 - Rapid cDNA Screening Service Life Science & Biotechnology No.8 - 43 - Rapid cDNA Screening Service Life Science & Biotechnology No.8 - 44 - Sac I T7 T3 Sma I Life Science & Biotechnology No.8 - 45 - Life Science & Biotechnology No.8 - 46 - Life Science & Biotechnology No.8 - 47 - Life Science & Biotechnology No.8 - 48 - Life Science & Biotechnology No.8 - 49 - Life Science & Biotechnology No.8 - 50 - Life Science & Biotechnology No.8 - 51 - Life Science & Biotechnology No.8 - 52 - ...
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This note was uploaded on 08/01/2009 for the course 221 22412 taught by Professor Maz during the Spring '09 term at A.T. Still University.

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