PCR 특집 Series7 - 2차구ì¡&d

PCR 특집 Series7 - 2차구ì¡&d

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Life Science & Biotechnology ઁ7ഐ (ઁ 3ӂ 2ഐ) 1998֙ 6ਘ 10ੌ ಞ૘ੋ ੉ઁഅ ߊ೯ੋ ੉ઁഅ ߊ೯୊ ࠁೠ߄੉য়ݫ٣ண(઱) ъթҳ بҋ 2ز 451-3 <੹ޙ؀ܻ੼> (઱)֣भ੗ন೯ L/S ࢎস౱ Tel.02-3471-7437 Fax.02-581-0136 E-mail : [email protected] [email protected] (઱)௏ই߄੉য়दझమ Tel. 02-841-7530 Fax.02-841-7531 0331-284-8592( ࣻਗ ) 042-622-2726( ؀੹ ) E-mail : [email protected] TAKARA-KOREA BIOMEDICAL INC. Technical Support Line Tel.02-577-2002 Fax.02-577-3691 E-mail [email protected] URL www.biochem.or.kr/bohanbio.htm ౠ૘ 1 PCR ౠ૘ दܻૉ 7 2ର ҳઑܳ औѱ ഋࢿೞח RNA੄ PCR 2 ౠ૘ 2 Gene Targetingਊ Recombinant ES Cell ઁ੘ 4 ౠ૘ 3 ߓনࣁನ੄ ੹୓ N -glycan੄ ҳઑ೧ࢳ 8 ো੤ӝദ PCR ӝୡъઝ 6 PCRਸ ੉ਊೠ DNA, RNA ೧ࢳӝࣿ੄ ਗܻ 12 Technical Tips 1. ഋҟಞҟب ஏ੿दझమ Full-Range BEACON 2000 20 2. TaKaRa PCR Mycoplasma Detection Setਸ ੉ਊೠ 22 Mycoplasma bovis ੄Ѩ୹ ױߔ૕ োҳਊ दড ࣗѐ (3) 23 ઱ݾ࢚ಿ 1. Solid Phase cDNA Synthesis Kit 26 2. cDNA PCR Library Kit 28 3. PerfectShot Ex Taq (Loading dye mix) 30 4. DNA-OFF, RNase-OFF 31 5. Dr. GenTLE(ബݽ, Ӓ۔ নࢿӐਊ) 32 6. Positive Selection Vector pGATA 33 7. SCDase(Sphingolipid Ceramide -deacylase) 34 8. Precoat-type E-cadherin EIA Kit 36 9. CFLP Scanning System 38 TaKaRa Custom Service 5 - ױߔ૕ ߂ peptide੄ ࠙੗۝ ஏ੿ - 11 प೷ъઝ ݶ৉ ઑ૒ ച೟੸ ৏࢝ߨ੄ ӝୡ (1) - ઑ૒੺ಞ - 40 Novagenࢎ ઁಿ ࣗѐ 42 Bender Med System - Apoptosis োҳਊ ઁಿ - 45 नઁಿ উղ 47 ੘਷ উղ ௾ ࣗध 19
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2ର ҳઑܳ औѱ ഋࢿೞח RNA੄ PCR Bca BEST TM RNA PCR Kit Ver. 1.1 Life Science & Biotechnology No. 7 2 1 Version upਵ۽ ࠂ੟ೠ 2ରҳઑ੄ RT-PCR੉ ਊ੉೤פ׮. Bca BEST TM RNA PCR Kitח ࠂ੟ೠ 2ର ҳઑܳ ыח mRNAب ֫਷ ബਯ۽ cDNA۽ ߸ജೡ ࣻ ੓ח ղৌࢿ DNA ೤ࢿബࣗ BEST DNA Polymerase(৉੹ࢎ߈਽ ୭੸ৡبо 65Ø)৬ ૐ಩ബਯ੉ ೱ࢚ೞب۾ ѐࢶೠ Bca-optimized Taq TM ਸ ଻ਊೠ RT-PCR kit੉׮. ࠄ Ҋীࢲח Әߣ ߈਽ઑѤ ١ਸ ߸҃ೞৈ ࠂ੟ೠ 2ର ҳઑܳ ыח ઱ഋਸ ૐ಩ೡ ࣻ ੓ب۾ ѐ۝ೠ ઁಿਸ ӝઓ੄ ઁಿҗ ׼ࢎ੄ ׮ܲ RT-PCR kit৬ ࠺Үೠ Ѿҗܳ ࣗѐೞҊ, ژೠ ࠄ ః౟ܳ ࢎਊೞৈ ࠁ׮ ࠂ੟ೠ 2ରҳઑܳ ыח ઱ ഋਸ ૐ಩ೡ ٸ੄ ߈਽ઑѤਸ ࣗѐೠ׮. õ Version upਵ۽ ׳ۄ૓ ղਊ [৉੹ࢎ߈਽ઑѤ] ߸҃੹ : 65Ø 1࠙ 30Ø 1࠙ Ä1 5 65Ø 15~30࠙ 80Ø 2࠙ 5࠙ [2ߓ 1st buffer੄ ਊ۝] ߸҃੹ : 500 ɋ [Control RNA, Control Primer] ߸҃੹ : HL60 ਬې total RNA(1 ɠ/ɋ) TFR ৔৉ 964 bpܳ ૐ಩ೡ ࣻ ੓ח primerह ߸҃റ : pBR322 ਬې tetracyclin ղࢿਬ੹੗ܳ ыח 1.4 kb ੄ ױಞਸ SP6 promotor ৔৉੄ ೞܨী ࢗੑೠ plasmid pSTet3ܳ ઱ഋਵ۽ SP6 RNA polymerase ী੄೧ in vitro ীࢲ ৉੹ࢎೞৈ ೤ࢿೠ RNA(2 ¨10 5 copies/ɋ).
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PCR 특집 Series7 - 2차구ì¡&d

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