Efficient plant regeneration from cotyledon explants of bottle gourd ( Lagenaria siceraria Standl.)

Efficient plant regeneration from cotyledon explants of bottle gourd ( Lagenaria siceraria Standl.)

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Plant Cell Rep (2004) 23:291–296 DOI 10.1007/s00299-004-0846-3 CELL BIOLOGY AND MORPHOGENESIS J-S. Han · D-G. Oh · I-G. Mok · H-G. Park · C. K. Kim Efficient plant regeneration from cotyledon explants of bottle gourd ( Lagenaria siceraria Standl.) Received: 12 December 2003 / Revised: 28 June 2004 / Accepted: 29 June 2004 / Published online: 10 September 2004 # Springer-Verlag 2004 Abstract Using cotyledon explants excised from seed- lings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd ( Lagenaria siceraria Standl.). Maximum shoot regenera- tion was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO 3 under a 16-h pho- toperiod. After 3–4 weeks of culture, 21.9–80.7% of ex- plants from the five cultivars regenerated shoots. Ad- ventitious shoots were successfully rooted on a half- strength MS medium with 0.1 mg/l IAA for 2–3 weeks. Flow cytometric analysis revealed that most of the re- generated plants derived from culture on medium with AgNO 3 were diploid. Keywords Bottle gourd ( Lagenaria siceraria Standl.) · Regeneration · Cotyledon · Silver nitrate · Ploidy Abbreviations AVG: l - a -(2-Aminoethoxyvinyl) glycine · BA: 6-Benzylaminopurine · IAA: Indole-3-acetic acid · MS: Murashige and Skoog’s · NAA: 1-Naphthaleneactic acid Introduction The bottle gourd ( Lagenaria siceraria Standl.), as all other gourds, belongs to Cucurbitaceae. It probably orig- inated in tropical Africa and now has a pantropical dis- tribution. It is a monoecious annual vine with a long, ribbed stem and strong tendrils. The mature fruit of bottle gourd is not a highly desirable food as it has little flesh and an offensive smell with a bitter taste. Its shell, how- ever, becomes tough when it matures and makes it at- tractive for handicrafts. It is interesting that this species is exclusively used as a rootstock for watermelon and squash, especially for production in the winter green- house, because it possesses excellent tolerance to low soil temperature and to soil-borne pathogens such as Fusari- um oxysporum f. sp. niveum . The cultivation of grafted vegetables has been successfully practised in many Asian countries for several decades and it is increasing in Eu- rope (Lee and Oda 2003). Genetic improvement of bottle gourd has been mainly achieved by conventional plant breeding methods, especially F 1 hybrid breeding. More recent techniques in plant genetic engineering have ad- vanced and opened a new avenue for crop improvement. A system for plant regeneration from individual cells or explants, is essential for the application of genetic engi- neering of bottle gourd. There have been reports of regeneration in other spe-
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This note was uploaded on 08/01/2009 for the course 2231 22444 taught by Professor Park during the Spring '09 term at A.T. Still University.

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Efficient plant regeneration from cotyledon explants of bottle gourd ( Lagenaria siceraria Standl.)

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