Regeneration of Transgenic Rose (Rosa hybrida) Plants from Embryogenic Tissue

Regeneration of Transgenic Rose (Rosa hybrida) Plants from Embryogenic Tissue

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LOGIN nature.com MY ACCOUNT E-ALERT SIGN UP REGISTER SUBSCRIBE SEARCH ADVANCED SEARCH PUBLICATIONS A-Z INDEX BROWSE BY SUBJECT This journal go Journal home > Archive > Table of Contents > Research > Abstract Journal home Advance online publication Current issue Archive Supplementary info Careers & Recruitment Press releases Supplements Focuses Conferences For authors Online submission Permissions For referees Free online issue About the journal Contact the journal Subscribe Advertising work@npg naturereprints About this site For librarians NPG Resources Bioentrepreneur Nature Reviews Drug Discovery Nature Nature Medicine Nature Genetics RESEARCH Bio/Technology 12 , 609 - 613 (1994) doi:10.1038/nbt0694-609 Regeneration of Transgenic Rose ( Rosa hybrida ) Plants from Embryogenic Tissue Ebrahim Firoozabady 1, * , York Moy 1 , Neal Courtney- Gutterson 1 1 1 DNA Plant Technology Corporation, 6701 San Pablo Ave. Oakland, California 94608. * Corresponding author. Friable embryogenic tissues (FET) were recovered from filament cultures of rose, Rosa hybrida cv. Royalty, and cocultured with Agrobacterium tumefaciens or A. rhizogenes harboring a vector containing neomycin phosphotransferase II (NPTII) and - glucuronidase (GUS) or firefly luciferase (LUC) genes. Putative transformed colonies were selected on kanamycin. Fifty 60 transformed embryogenic callus lines were reproducibly obtained from each gram of FET inoculated with Agrobacterium . Transformed embryogenic calli were transferred to maturation media to form somatic embryos, which subsequently produced flowering plants. The transgenic nature of the plants was confirmed by enzyme assays, polymerase chain reaction, and Southern hybridization. Over 100 transgenic plants have been established in soil and flowered in the greenhouse. This procedure facilitates introduction of desirable genes, especially those controlling flower color, into commercial cultivars of rose. ABSTRACT
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Regeneration of Transgenic Rose (Rosa hybrida) Plants from Embryogenic Tissue

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