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Unformatted text preview: Chapter 14 Electron Transport and Oxidative Phosphorylation Oxidative phosphorylation - process in which NADH and QH 2 are oxidized and ATP is produced. Enzymes are found in inner mitochondrial membrane in eukaryotes. In prokaryotes, enzymes are found in cell membrane. Process consists of 2 separate, but coupled processes: 1) respiratory electron-transport chain Responsible for NADH and QH 2 oxidation Final e- acceptor is molecular oxygen Energy generated from electron transfer is used to pump H + into intermembrane space from matrix ---> matrix becomes more alkaline and negatively charged. 2) ATP synthesis Proton concentration gradients represents stored energy When H + are moved back across inner mitochondrial membrane through ATP synthase ---> ADP is phosphorylated to form ATP Chemiosmotic Theory of ATP Production Proposed by Peter Mitchell in 1961 (won Nobel Prize for this work). Tenet: Proton concentration gradient serves as energy reservoir for ATP synthesis. Proton concentration gradient also known as proton motive force (PMF). Components of Electron Transport System There are 5 protein complexes: I) NADH-ubiquinone oxidoreductase II) succinate-ubiquinone oxidoreductase III) ubiquinol-cytochrome c oxidoreductase IV) cytochrome c oxidase V) ATP synthase Electrons flow through ETS in direction of increasing reduction potential....
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This note was uploaded on 08/02/2009 for the course BIOCHEMIST 100 taught by Professor Crew during the Spring '05 term at North Alabama.
- Spring '05