Unformatted text preview: o Steady state and quasi steady state o Equilibrium and quasi equilibrium 2. Enzyme kinetics o Derivation and application of Michaelis-Menten kinetics o Able to linearize MM kinetics in different forms o Calculation of initial rates based on enzyme concentration, substrate concentration, and Km o Able to use simplify MM kinetics depending on the specific parameters and initial conditions (for instance, MM is zeroth order in [S] if [S]>>Km, and it is the first order if [S]<<Km) o Inhibition of Michaelis-Menten kinetics o Calculation of optimal substrate concentration in the case of substrate inhibition During Exam: 1) Strategize your time. Finish the easy problems first. 2) Write down your assumptions in your derivation of calculation clearly. You may get partial credits even if you can’t provide completely correct answer for the problem....
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- Spring '07
- Michaelis-Menten kinetics, simplify MM kinetics