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Eukaryotes mrna processing after transcription

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Unformatted text preview: yotes: mRNA processing after transcription produces a mature mRNA 1. adding a methylated cap at the 5' end and poly-A tail at the 3' end 2. RNA splicing removes sequences known as introns from the primary transcript 3. alternative splicing often produces different mRNAs from the same primary transcript III. Translation: base-pairing between mRNA and tRNAs directs assembly of a polypeptide on the ribosome A. tRNAs mediate the translation of of mRNA codons to amino acids 1. tRNA structure: a compact "L" carrying an anticodon at one end and an amino acid at the other 2. base-pairing between a mRNA codon and a tRNA anticodon directs incorporation of an amino acid into a growing polypeptide 3. Wobble: some tRNAs can recognize more than one codon B. Ribosomes are the sight of polypeptide synthesis 1. complex structures composed of RNA and protein 2. different parts of the ribosome have different functions C. Mechanism of translation: differences between eukaryotes and prokaryotes D. Processing after translation can change a polypeptide's structure IV. Comprehensive example: a computerized analysis of gene expression in C. elegans V. How mutations affect gene expression A. Mutations in a gene's coding sequence can alter the gene product...
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