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Chapter+1+lecture+outline - Chapter 8- Transcription,...

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Chapter 8- Transcription, Translation, and Bioinformatics RNA Synthesis Transcription- making an DNA- made of Sugar- RNA- made of Sugar- Three types of RNA: 1. ____________ Contains _________________________________ 2. ____________ a. Carries __________________ to build the polypeptide during _______________ 3. ____________ a. Component of Template strand- DNA strand that is being copied into RNA polymerase- enzyme that Bacterial RNA polymerase 4 subunits: Process of transcription 3 phases: - - - Transcription of RNA from DNA involves the enzyme RNA polymerase , which adds ribonucleotides onto the 3' ends of growing RNA chain. Unlike DNA polymerase, RNA polymerase needs no RNA polymerase recognizes a specific start sequence on the DNA called the Transcription Initiation ____________________________________ RNA polymerase Forms RNA polymerase ____________________________ RNA polymerase binds ___________________________ Sequence on DNA 10 and 35 bases upstream of start site Polymerase ____________________________________ at promoter 1
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Sigma factor _______________________ Promoters Specific DNA sequences indicating start site of transcription Two important regions: (in Bacteria ) -35 sequence -10 sequence (Pribnow box) (- means upstream of start site) Prokaryotes have a ___________________________________ with a _____________________________________ . The sigma factor binds to the _________________________________________________________. Multiple sigma factors __________ Guides RNA polymerase to most genes __________ Active when cell is _________________ response Transcription Elongation Core RNA polymerase moves down the template DNA strand from 3’ to 5’ RNA strand is made 5’ to 3’ Transcription Termination When RNA polymerase reaches Polymerase slows at pause site 2
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____________________________ termination Rho ( r ) factor _______________ along mRNA to __________________
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This note was uploaded on 08/04/2009 for the course BIOL 2051 taught by Professor Brininstool during the Fall '07 term at LSU.

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Chapter+1+lecture+outline - Chapter 8- Transcription,...

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