9 - PROKARYOTIC DIVERSITY BIOL 4125 SPRING 2009 LECTURE 9...

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PROKARYOTIC DIVERSITY BIOL 4125 SPRING 2009 LECTURE 9 The Proteobacteria Part 1
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Norman Pace The number of bacterial phyla known has increased ~10-fold in the last 20 years
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Although the number of bacterial phyla with cultured representatives has tripled in the last two decades, many of these contain only a few representative strains. The physiology and metabolism of ~80% of bacterial phyla remain unknown.
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The Proteobacteria is by far the largest group in all the Bacteria An unrooted phylogenetic tree of Proteobacteria and related bacteria. Tree is based on the phylogeny of 30 core proteins. Bern & Goldberg (2005) BMC Evolutionary Biology 5:34 The Proteobacteria consist of 5 clusters and numerous genera. Physiologically, they can be either phototrophic, chemolithotrophic, or chemoorganotrophic.
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The cell requires NAD(P)H for CO 2 fixation. Thus, when growing on poor electron donors such as H 2 S, NO 2 - , or Fe 2+ , energy must be used to drive electrons against a thermodynamic gradient. To grow autotrophically, ATP production is not enough CO 2 (CH 2 O) n ATP ADP NAD(P)H NAD(P) + Exergonic Endergonic
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Reverse electron transfer: energy-dependent movement of electrons against a thermodynamic gradient to form NADH electron donors (AH 2 ) of extremely positive E 0 AH 2 A • Electrons from AH 2 are funneled into the respiratory chain and used to generate a PMF. • The PMF is used to
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This note was uploaded on 08/04/2009 for the course BIOL 4125 taught by Professor Christner during the Spring '08 term at LSU.

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9 - PROKARYOTIC DIVERSITY BIOL 4125 SPRING 2009 LECTURE 9...

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