Huber_etal1998_ThermocrinisYNP - A PPLIED AND ENVIRONMENTAL...

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Unformatted text preview: A PPLIED AND ENVIRONMENTAL MICROBIOLOGY, 0099-2240/98/$04.00 1 Oct. 1998, p. 35763583 Vol. 64, No. 10 Copyright 1998, American Society for Microbiology. All Rights Reserved. Thermocrinis ruber gen. nov., sp. nov., a Pink-Filament-Forming Hyperthermophilic Bacterium Isolated from Yellowstone National Park ROBERT HUBER, 1 * WOLFGANG EDER, 1 STEFAN HELDWEIN, 1 GERHARD WANNER, 2 HARALD HUBER, 1 REINHARD RACHEL, 1 AND KARL O. STETTER 1 Lehrstuhl fu r Mikrobiologie and Archaeenzentrum, Universita t Regensburg, D-93053 Regensburg, 1 and Botanisches Institut, Universita t Mu nchen, D-80638 Munich, 2 Germany Received 4 May 1998/Accepted 23 July 1998 A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occur- ring in the upper outflow channel (temperature, 82 to 88C) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89C in the neutral to alkaline pH range. Depending on the culture conditions, the organisms occurred as single motile rods, as aggregates, or as long filaments that formed streamer-like cell masses. The novel isolate grew chemolithoautotrophically with hydrogen, thiosulfate, and elemental sulfur as electron donors and oxygen as the electron acceptor. Alterna- tively, under aerobic conditions, formate and formamide served as sole energy and carbon sources. The novel isolate had a 16S rRNA sequence closely related to the 16S rRNA sequence obtained from uncultivated pink filaments. It represents a new genus in the order Aquificales , the type species of which we name Thermocrinis ruber (type strain, OC 1/4 [ 5 DSM 12173]). Currently, the only chemolithoautotrophic, hyperthermo- philic representatives in the bacterial domain are members of the genus Aquifex , which grow at optimal and maximal tem- peratures of 85 and 95C, respectively (23, 43). The type strain of Aquifex pyrophilus was originally isolated from a submarine hydrothermal vent system at the Kolbeinsey Ridge north of Iceland at a depth of 106 m. In the energy-yielding reactions of this organism hydrogen, elemental sulfur, and thiosulfate serve as electron donors, while oxygen and nitrate are used as elec- tron acceptors (23). Therefore, Aquifex pyrophilus is a faculta- tive aerobe. Phylogenetic analyses based on 16S rRNA se- quence comparisons showed that Aquifex pyrophilus represents the deepest divergence in the bacterial domain (17). Due to this outstanding phylogenetic position, the new order Aquifi- cales was proposed. The closest known relatives of the genus Aquifex are members of the genus Hydrogenobacter , which be- long to the same order (17, 36). Representatives of the genus Hydrogenobacter grow optimally at temperatures between 70 and 75C, while no growth is observed at 81C (3, 7). They are autotrophic aerobes, growing by oxidation of hydrogen, thio- sulfate, and elemental sulfur (1, 3, 7, 8). Hydrogenobacter ther- mophilus , Calderobacterium hydrogenophilum...
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This note was uploaded on 08/04/2009 for the course BIOL 4125 taught by Professor Christner during the Spring '08 term at LSU.

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Huber_etal1998_ThermocrinisYNP - A PPLIED AND ENVIRONMENTAL...

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