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sp07 second exam key

sp07 second exam key - Metabolic Biochemistry BIBC 102...

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Unformatted text preview: Metabolic Biochemistry / BIBC 102 Second Exam / Spring 2007 I. (20 points) You are given a preparation of intact mitochondria in a suspension containing plentiful amounts of oxygen, phosphate, ADP, and malonate, a competitive inhibitor of succinate dehydrogenase. Under these conditions, all TCA eyele intennediates are converted to succinate, which builds up in the mitochondria. In your experiment, you add pyruvate labeled with l“C in the methyl carbon (”CHg-C(O)— cog) to this mitochondrial suspension, incubate, and then isolate succinate. Show how you can synthesize succinate starting from pyruvate alone. (This means that you are given a pool of pyruvate molecules and that all carbon atoms in TCA intermediates must be made from these molecules.) Draw the correct structure for each intermediate molecule. Include the names or abbreviations of any other reactants and products (eg, ATP, ADP, Pi, NAD-t, NADH, etc), and any required prosthetic groups (eg, biotin, TPP,etc.).Andtracethe fate otthe“ .. uccinate. MW 2 II. (25 points) Fill in all of the enzyme catalyzed reactions which convert the substrate on the upper left to the product on the lower right. Draw the correct structure for each intermediate molecule, except for carnitine (which can be shown as a box with an OH projecting off of it). Include the names or abbreviations of any other reactants and products (e.g., ATP, ADP, Pi, NAD+, FAD, NADH, etc.), any required cofactors (e.g., biotin, 1*“ ‘ s ), and indicate 8 - ular location so] or matri @ o t" ' so é?) ”VS '0 I A. CH3(CH2)143-0 (0310301) +- o o CH3(CH2)13 ('5 SCoA + CH3 3 SCoA matrix} (matrix) ease indicate which one of the above enzymatic reactions is under allosteric regulation, the identity of the allosteric activators and/or inhibitors of this e e, and the physiological role of this regulation in a cell. 414 71' x3 sad/e gkva/é EA; 1.7;:4/ b; m A»?! Q ‘4’ Al. ‘ U18», 1/ $13.5 #473 1%; _/‘t'ah/A/'-;AJ IIUAIZI' ,1: Q2/ mZ/‘fia; A7 pmmqm~£zégazssa¥§mlfiw_,m{gages—w ”it- ... 7* L015") )C “(2/ 45; 33$ fl," “:4.“ , {.3 ’6. - ,. _ ..,....—m- “_c.“___ #15“. ......, 3 III. (25 points) (@ diagram of the overview of proton and electron circuits in mitochondria starting with NADH in the matrix and ADP and P, in the cytosol and ending with the reduction of oxygen to water and the formation of ATP (the diagram in the lecture handout will receive full credit). Label each complex and protein, identify matrix, intermembrane space, and inner membrane, and indicate the flow of protons and W3 r Describe (or illustrate with a diagram) how electron transport is coupled to the formation of ATP, ; and briefly explain how this coupling accounts for the increased 0; consumption during aerobic exercise. WM” ‘l ”Av-5‘ ”ff #:ko _) ‘4____._._- AM 5 flirt—An... [xmz'se INCMAIP: [El-019‘}, d’zu‘eé 4/47“): #759 SLIM-flare A mantle Mm ”776 L/l/c-Z A/A’J'LJJ eke/.1... Anus/2W A rue/are Mm: 0:. A #10, Q 4 IV. (10 points) Answer two of the following three parts (A to C); cross out the part that ou don t wish to have graded or we will cross out part A for you. . Mitchell and Moyle carried out an elegant series of experiments using a sensitive pH meter to measure ‘3 anges in hydrogen ion concentration in an anaerobic suspension of rat mitochondria. Explain each of the llowing observations: 1. The anaerobic suspension was incubated with hydroxybutyrate, a source of NADH in the mitochondrial matrix. When a small amount of oxygen was then injected into the suspension, an immediate decrease in pH was observed. 2. When this experiment was carried out in the presence of Triton I, a detergent that disrupted membranes, no decrease in pH was observed when oxygen was injected into the suspension. ll “M! 139.96% 4 ‘92.. aflawz/ e/relév—S 74’ 5‘ AW’W 9w W# in 0;) (JAJ 1422?; 24¢ flM—wf/Ua o‘F fP'olLuuS 'F—u-nq fin-e {HA—AIL! /;‘VAD At Suffpnflz) (ht/”um amid So (are; ft: {9/9 )4: 142/. CE 7 7: {Le xfiéf-‘aedr I/é’r‘n/A—o/ K1 /;J/é?/‘I% AF A a/vjea/ wan/4r- MI-m )7”. Me CWA-r‘hnrfi' aF IQ N24 Josh's, mm, fl tharx‘J/e A, Q effié/ILM K:— fl/‘bA/J jfio/rédffi . You are given purified, intact mitochondria, an abundant source of matrix NADH, excess ADP, and plentiful ' hosphate. You now add exactly 10 micromoles of 02110 this system and measure the changes in ATP concentration over time until no more 02 is present. How many micromoles of ATP will be eventually formed? (Show your calculations: base our calculations on the uantitative flow of rotons across the inner a = I W M .1 L. . Certain branched chain fatty acids such as pristanic acid can undergo oxi tion. (a) How many cyc es of B x idation are required to completely degrade this fatty acid? (b) How many C2 (acetyl CoA). C3 (propionyl CoA), and Cl? ( methylpropionyl CoA) products are formed? 0. CH3, 0 .1’ . -. CH3 —6 él-l Edit-CHt-CHL 9—dfl —é' —-5 C. @6 -a7c[¢$ a; F140 ...
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