BIBC 102 handout 5 (10_8-10_10)

BIBC 102 handout 5 (10_8-10_10) - BIBC 102 Coleman Section:...

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BIBC 102 Coleman JP Abraham Section: B05 Monday 3:00p - 3:50p CENTR 218 jmabraha@ucsd.edu Handout 5 (10/8-10/10) Enzyme commission gives each enzyme a 4 digit number (EC number) 1 st digit of EC number can be 1 - 6 o 1 ~ oxidoreductases: catalyze electron transfer reactions o 2 ~ transferases: move chemical groups between substances ex. kinase: transfers phosphates o 3 ~ hydrolases: cut with water o 4 ~ lyases: create/remove double bond by add/removing chemical groups from carbon atom o 5 ~ isomerases: move chemical group on single substrate molecule ex. triose phosphate isomerase: moves carbonyl group o 6 ~ ligases: form single bonds usually between 2 different substrates (ex. C-C, C-S, C-O, etc); considered condensation reaction nomenclature example: hexokinase (first enzyme in glycolysis) reaction catalyzed: ATP + glucose ADP + G6P EC number: 2.7.1.1 o 2 = transferase; 7 = phosphotransferase; 1 = OH is acceptor of P; 1 = d glucose (naturally occurring glucose isomer in the body) is the substrate that receives the phosphate enzyme helper molecules cofactor: required for a reaction to proceed o metal ion cofactor metals ions
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BIBC 102 Coleman JP Abraham Section: B05 Monday 3:00p - 3:50p CENTR 218 jmabraha@ucsd.edu Handout 5 (10/8-10/10) ex. Cu ion, Mg ion, Ni ion, Fe ion o coenzymes small organic molecules ex. NAD+/NADH prosthetic group: categorized as a coenzyme coenzyme that is attached to enzyme enzyme/coenzyme nomenclature: holoenzyme - enzyme + cofactor apoenzyme - enzyme alone (without its cofactor) In 1913, Leonor Michaelis and Maud Menten came up with general theory of enzyme action [Enzyme] + [Substrate] k -1 k 1 [ES] k 2 (k cat ~ slow step) [E + Product] V o Initial velocity of the reaction Looks at rxn only in forward direction What effects V o ? o Substrate concentration
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JP Abraham Section: B05 Monday 3:00p - 3:50p CENTR 218 jmabraha@ucsd.edu Handout 5 (10/8-10/10) Vo is specific for an enzyme concentration o For example, V o for [S] = 0.2 mM at [E] = 0.1 mM is NOT THE SAME as V o for [S] = 0.2 mM at [E] = 0.3 mM MM equation: V o = V max x [substrate] (K m ) + [substrate] MM graph illustration: What do the terms of the equation represent? V
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BIBC 102 handout 5 (10_8-10_10) - BIBC 102 Coleman Section:...

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