BIBC 102 handout 9 (10-27)

BIBC 102 handout 9 (10-27) - BIBC 102 Coleman Section: B05...

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BIBC 102 Coleman JP Abraham Section: B05 Monday 3:00p - 3:50p CENTER 218 jmabraha@ucsd.edu Handout 9 (10/27) OH at ROMA after section 3. phosphofructokinase-1 (PFK 1) F6P 1,6 FBP ATP ADP + Pi per F6P o at this point, a net 2 ATP have been broken down The reaction is irreversible and exergonic 4. aldolase 1,6 FBP  DHAP + G3P ∆G*’ = 22.8, but under cellular conditions, ∆G is closer to 0 This means that [P] and [R] determine direction of reaction and the reaction is REVERSIBLE 5. TPI DHAP  G3P ∆G = ~ 0 This means that [P] and [R] determine direction of reaction and the reaction is REVERSIBLE Note: TPI is an “isomerase” DHAP and G3P are ISOMERS! 6. G3P dehydrogenase G3P  1,3 BPG Summary: o H- is transferred to another substrate here (note: we are looking at a dehydrogenase) H- from the aldehyde group of G3P is put onto NAD+, making NADH o Pi is added onto G3P as well This makes a high energy acyl phosphate molecule, with a high energy
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BIBC 102 handout 9 (10-27) - BIBC 102 Coleman Section: B05...

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