BIBC 102 handout 11 (11-3-11-7)

BIBC 102 handout 11 (11-3-11-7) - BIBC 102 Coleman Section:...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
BIBC 102 Coleman JP Abraham Section: B05 Monday 3:00p - 3:50p CENTER 218 [email protected] Handout 11 (11/3 – 11/7) OH at ROMA after section Cellular resp – requires O2 spits out CO2 O2 is used in mitochondria made into H2O CO2 is made in TCA cycle O2 is NOT CONVERTED to CO2 Enxymes: refer to TCA cycle handout for details citrate synthase o reaction: oxaloacetate citrate o condensation reaction o Acetyl group from acetyl-CoA is added to oxaloacetate aconitase o reaction: citrate isocitrate o moves OH from one carbon on citrate to another carbon on isocitrate ISOMERIZATION RXN o NOTE: intermediate forms in between citrate and isocitrate isocitrate dehydrogenase o reaction: isocitrate alpha-ketoglutarate o 1. oxidation of isocitrate NAD+ accepts H- to make NADH on OH OH is turned into carbonyl group ( C=O )
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
BIBC 102 Coleman JP Abraham Section: B05 Monday 3:00p - 3:50p CENTER 218 [email protected] Handout 11 (11/3 – 11/7) OH at ROMA after section o 2. CO2 on C3 is lost alpha-ketoglutarate dehydrogenase o reaction: alpha-ketoglutarate succinyl-CoA o 1. CoA attached (forms a high energy bond) o 2. CO2 lost o 3. NAD+ NADH o exergonic reaction (-33.5 kJ/mol) succinyl-CoA synthetase o reaction: succinyl-CoA succinate o note: succinyl-CoA is a high energy intermediate in theTCA cycle o 1. breaking the thioester bond supplies energy for… o 2. GDP GTP o note: O added to succinate comes from Pi o Why is GTP important? GTP + ADP ATP + GDP ∆G = 0 nucleoside diphosphate kinase THIS IS A SUBSTRATE LEVEL PHOSPHORYLATION! o NOTE: molecule becomes symmetrical at this point (refer to Ronnie’s TCA cycle handout) succinate dehydrogenase o Reaction: succinate fumerate
Background image of page 2
BIBC 102 Coleman JP Abraham Section: B05 Monday 3:00p - 3:50p CENTER 218 [email protected] Handout 11 (11/3 – 11/7) OH at ROMA after section o FAD FADH2 FADH2 goes directly into the electron transport chain (at complex 2… FADH2 skips complex 1 in the electron transport chain) o note: this enxyme is bound to inside of mitochindria at the inner mitochindrial membrane fumarase o reaction: fumerate malate o hydraton step malate dehydrogenase o malate oxaloacetate o NAD+ NADH 2.5 ATP/NADH 1.5 ATP/FADH2 1 ATP/GTP Summary: glycolysis to TCA cycle… Glucose 2 pyr o 2 ATP = 2 ATP o 2 NADH = 3 ATP (because they are made in the cytosol, each NADH must use one ATP to get into the matrix) 2 pyr 2 acetyl-CoA o 2 NADH = 5 ATP 2 acetyl-CoA in TCA cycle
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 08/05/2009 for the course BIBC 102 taught by Professor Price during the Fall '02 term at UCSD.

Page1 / 10

BIBC 102 handout 11 (11-3-11-7) - BIBC 102 Coleman Section:...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online